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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella typhimurium DnaJ-like protein DjlA (djlA), partial CSB-YP759000SXB
CSB-EP759000SXB
CSB-BP759000SXB
CSB-MP759000SXB
CSB-EP759000SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP759001SMP
CSB-EP759001SMP
CSB-BP759001SMP
CSB-MP759001SMP
CSB-EP759001SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Homoserine O-succinyltransferase (metA) CSB-YP759002AYS
CSB-EP759002AYS
CSB-BP759002AYS
CSB-MP759002AYS
CSB-EP759002AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Amino acid--[acyl-carrier-protein] ligase (Atu2573) CSB-YP759003AYS
CSB-EP759003AYS
CSB-BP759003AYS
CSB-MP759003AYS
CSB-EP759003AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Heme A synthase (ctaA), partial CSB-YP759004AYS
CSB-EP759004AYS
CSB-BP759004AYS
CSB-MP759004AYS
CSB-EP759004AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP759005AYS
CSB-EP759005AYS
CSB-BP759005AYS
CSB-MP759005AYS
CSB-EP759005AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Long-chain-fatty-acid--CoA ligase FadD19 (fadD19), partial CSB-YP759006MVZ
CSB-EP759006MVZ
CSB-BP759006MVZ
CSB-MP759006MVZ
CSB-EP759006MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized PPE family protein PPE49 (ppe49) CSB-YP759007MVZ
CSB-EP759007MVZ
CSB-BP759007MVZ
CSB-MP759007MVZ
CSB-EP759007MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alpha-pyrone synthesis polyketide synthase-like Pks18 (pks18) CSB-YP759008MVZ
CSB-EP759008MVZ
CSB-BP759008MVZ
CSB-MP759008MVZ
CSB-EP759008MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional regulatory protein tcrA (tcrA) CSB-YP759009MVZ
CSB-EP759009MVZ
CSB-BP759009MVZ
CSB-MP759009MVZ
CSB-EP759009MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative uncharacterized protein ydet (ydeT) CSB-YP759010EOD
CSB-EP759010EOD
CSB-BP759010EOD
CSB-MP759010EOD
CSB-EP759010EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Putative lipoprotein NMB1124/NMB1162 (NMB1124) CSB-YP759011NGG
CSB-EP759011NGG
CSB-BP759011NGG
CSB-MP759011NGG
CSB-EP759011NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B UPF0434 protein NMB0674 (NMB0674) CSB-YP759012NGG
CSB-EP759012NGG
CSB-BP759012NGG
CSB-MP759012NGG
CSB-EP759012NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B 30S ribosomal protein S17 (rpsQ) CSB-YP759013NGG
CSB-EP759013NGG
CSB-BP759013NGG
CSB-MP759013NGG
CSB-EP759013NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bleomycin resistance protein (ble) CSB-YP759014FKZ
CSB-BP759014FKZ
CSB-MP759014FKZ
CSB-EP759014FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Calmodulin-5/6/7/8 (PCM5) CSB-YP759015FIG
CSB-EP759015FIG
CSB-BP759015FIG
CSB-MP759015FIG
CSB-EP759015FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cellulose synthase-like protein D3 (CSLD3), partial CSB-YP759016OFG
CSB-EP759016OFG
CSB-BP759016OFG
CSB-MP759016OFG
CSB-EP759016OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Secretory carrier-associated membrane protein 2 (SCAMP2), partial CSB-YP759017OFG1
CSB-EP759017OFG1
CSB-BP759017OFG1
CSB-MP759017OFG1
CSB-EP759017OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica BURP domain-containing protein 13 (BURP13) CSB-YP759018OFG
CSB-EP759018OFG
CSB-BP759018OFG
CSB-MP759018OFG
CSB-EP759018OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable UDP-arabinopyranose mutase 2 (UAM2) CSB-YP759019OFG
CSB-EP759019OFG
CSB-BP759019OFG
CSB-MP759019OFG
CSB-EP759019OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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