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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio vulnificus D-alanine--D-alanine ligase (ddl) CSB-YP759253VCQ
CSB-EP759253VCQ
CSB-BP759253VCQ
CSB-MP759253VCQ
CSB-EP759253VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Acetate kinase 2 (ackA2) CSB-YP759254VCQ
CSB-EP759254VCQ
CSB-BP759254VCQ
CSB-MP759254VCQ
CSB-EP759254VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB) CSB-YP759255VCQ
CSB-EP759255VCQ
CSB-BP759255VCQ
CSB-MP759255VCQ
CSB-EP759255VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Phosphate import ATP-binding protein PstB 2 (pstB2) CSB-YP759256VCQ
CSB-EP759256VCQ
CSB-BP759256VCQ
CSB-MP759256VCQ
CSB-EP759256VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Probable phosphatase VVA0289 (VVA0289) CSB-YP759257VCQ
CSB-EP759257VCQ
CSB-BP759257VCQ
CSB-MP759257VCQ
CSB-EP759257VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Pyrrolidone-carboxylate peptidase (pcp) CSB-YP759258VCQ
CSB-EP759258VCQ
CSB-BP759258VCQ
CSB-MP759258VCQ
CSB-EP759258VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0345 protein VVA0047 (VVA0047) CSB-YP759259VCQ
CSB-EP759259VCQ
CSB-BP759259VCQ
CSB-MP759259VCQ
CSB-EP759259VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus ATP synthase subunit alpha (atpA), partial CSB-YP759260VCQ
CSB-EP759260VCQ
CSB-BP759260VCQ
CSB-MP759260VCQ
CSB-EP759260VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Protein smg homolog (smg) CSB-YP759261VCQ
CSB-EP759261VCQ
CSB-BP759261VCQ
CSB-MP759261VCQ
CSB-EP759261VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Protein CrcB homolog (crcB), partial CSB-YP759262VCQ1
CSB-EP759262VCQ1
CSB-BP759262VCQ1
CSB-MP759262VCQ1
CSB-EP759262VCQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Pantothenate kinase (coaA) CSB-YP759263VCQ
CSB-EP759263VCQ
CSB-BP759263VCQ
CSB-MP759263VCQ
CSB-EP759263VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP759264VCQ
CSB-EP759264VCQ
CSB-BP759264VCQ
CSB-MP759264VCQ
CSB-EP759264VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Thiol:disulfide interchange protein DsbD (dsbD), partial CSB-YP759265VCQ
CSB-EP759265VCQ
CSB-BP759265VCQ
CSB-MP759265VCQ
CSB-EP759265VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Protein TusC homolog (tusC) CSB-YP759266VCQ
CSB-EP759266VCQ
CSB-BP759266VCQ
CSB-MP759266VCQ
CSB-EP759266VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP759267VCQ
CSB-EP759267VCQ
CSB-BP759267VCQ
CSB-MP759267VCQ
CSB-EP759267VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Bifunctional protein ArgH (argH), partial CSB-YP759268VCQ
CSB-EP759268VCQ
CSB-BP759268VCQ
CSB-MP759268VCQ
CSB-EP759268VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Alanine racemase (alr) CSB-YP759269VCQ
CSB-EP759269VCQ
CSB-BP759269VCQ
CSB-MP759269VCQ
CSB-EP759269VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP759270VCQ
CSB-EP759270VCQ
CSB-BP759270VCQ
CSB-MP759270VCQ
CSB-EP759270VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Probable Fe (2+)-trafficking protein (VV2885) CSB-YP759271VCQ
CSB-EP759271VCQ
CSB-BP759271VCQ
CSB-MP759271VCQ
CSB-EP759271VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Protein sprT (sprT) CSB-YP759272VCQ
CSB-EP759272VCQ
CSB-BP759272VCQ
CSB-MP759272VCQ
CSB-EP759272VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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