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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio vulnificus tRNA-modifying protein ygfZ (VV2842) CSB-YP759273VCQ
CSB-EP759273VCQ
CSB-BP759273VCQ
CSB-MP759273VCQ
CSB-EP759273VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP759274VCQ
CSB-EP759274VCQ
CSB-BP759274VCQ
CSB-MP759274VCQ
CSB-EP759274VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP759275VCQ
CSB-EP759275VCQ
CSB-BP759275VCQ
CSB-MP759275VCQ
CSB-EP759275VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Carbon storage regulator homolog (csrA) CSB-YP759276VCQ
CSB-EP759276VCQ
CSB-BP759276VCQ
CSB-MP759276VCQ
CSB-EP759276VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus DNA gyrase inhibitor YacG (yacG) CSB-YP759277VCQ
CSB-EP759277VCQ
CSB-BP759277VCQ
CSB-MP759277VCQ
CSB-EP759277VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP759278VCQ
CSB-EP759278VCQ
CSB-BP759278VCQ
CSB-MP759278VCQ
CSB-EP759278VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Ribosome-binding factor A (rbfA) CSB-YP759279VCQ
CSB-EP759279VCQ
CSB-BP759279VCQ
CSB-MP759279VCQ
CSB-EP759279VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Purine nucleoside phosphorylase DeoD-type 1 (deoD1) CSB-YP759280VCQ
CSB-EP759280VCQ
CSB-BP759280VCQ
CSB-MP759280VCQ
CSB-EP759280VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Na (+)-translocating NADH-quinone reductase subunit C (nqrC), partial CSB-YP759281VCQ1
CSB-EP759281VCQ1
CSB-BP759281VCQ1
CSB-MP759281VCQ1
CSB-EP759281VCQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 50S ribosomal protein L31 type B (rpmE2) CSB-YP759282VCQ
CSB-EP759282VCQ
CSB-BP759282VCQ
CSB-MP759282VCQ
CSB-EP759282VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP759283VCQ
CSB-EP759283VCQ
CSB-BP759283VCQ
CSB-MP759283VCQ
CSB-EP759283VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Hydroxyacylglutathione hydrolase 2 (gloB2) CSB-YP759284VCQ
CSB-EP759284VCQ
CSB-BP759284VCQ
CSB-MP759284VCQ
CSB-EP759284VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Cytochrome c biogenesis ATP-binding export protein CcmA (ccmA) CSB-YP759285VCQ
CSB-EP759285VCQ
CSB-BP759285VCQ
CSB-MP759285VCQ
CSB-EP759285VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP759286VCQ
CSB-EP759286VCQ
CSB-BP759286VCQ
CSB-MP759286VCQ
CSB-EP759286VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP759287VCQ
CSB-EP759287VCQ
CSB-BP759287VCQ
CSB-MP759287VCQ
CSB-EP759287VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0319 protein VV2327 (VV2327) CSB-YP759288VCQ
CSB-EP759288VCQ
CSB-BP759288VCQ
CSB-MP759288VCQ
CSB-EP759288VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Chromosome partition protein mukE (mukE) CSB-YP759289VCQ
CSB-EP759289VCQ
CSB-BP759289VCQ
CSB-MP759289VCQ
CSB-EP759289VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Protein TolB (tolB) CSB-YP759290VCQ
CSB-EP759290VCQ
CSB-BP759290VCQ
CSB-MP759290VCQ
CSB-EP759290VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0208 membrane protein VV2132 (VV2132), partial CSB-YP759291VCQ1
CSB-EP759291VCQ1
CSB-BP759291VCQ1
CSB-MP759291VCQ1
CSB-EP759291VCQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Biosynthetic arginine decarboxylase (speA), partial CSB-YP759292VCQ
CSB-EP759292VCQ
CSB-BP759292VCQ
CSB-MP759292VCQ
CSB-EP759292VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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