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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP759233WBQ
CSB-EP759233WBQ
CSB-BP759233WBQ
CSB-MP759233WBQ
CSB-EP759233WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit alpha (atpA) CSB-YP759234WBQ
CSB-EP759234WBQ
CSB-BP759234WBQ
CSB-MP759234WBQ
CSB-EP759234WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein grpE (grpE) CSB-YP759235WBQ
CSB-EP759235WBQ
CSB-BP759235WBQ
CSB-MP759235WBQ
CSB-EP759235WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor G (fusA), partial CSB-YP759236WBQ
CSB-EP759236WBQ
CSB-BP759236WBQ
CSB-MP759236WBQ
CSB-EP759236WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cysteine--tRNA ligase (cysS) CSB-YP759237WBQ
CSB-EP759237WBQ
CSB-BP759237WBQ
CSB-MP759237WBQ
CSB-EP759237WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arginine biosynthesis bifunctional protein ArgJ (argJ) CSB-YP759238WBQ
CSB-EP759238WBQ
CSB-BP759238WBQ
CSB-MP759238WBQ
CSB-EP759238WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 10 kDa chaperonin (groS) CSB-YP759239WBQ
CSB-EP759239WBQ
CSB-BP759239WBQ
CSB-MP759239WBQ
CSB-EP759239WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate--tRNA ligase 2 (gltX2) CSB-YP759240WBQ
CSB-EP759240WBQ
CSB-BP759240WBQ
CSB-MP759240WBQ
CSB-EP759240WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor Ts (tsf) CSB-YP759241WBQ
CSB-EP759241WBQ
CSB-BP759241WBQ
CSB-MP759241WBQ
CSB-EP759241WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP759242WBQ
CSB-EP759242WBQ
CSB-BP759242WBQ
CSB-MP759242WBQ
CSB-EP759242WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase (fabZ) CSB-YP759243WBQ
CSB-EP759243WBQ
CSB-BP759243WBQ
CSB-MP759243WBQ
CSB-EP759243WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis tRNA dimethylallyltransferase 1 (miaA1) CSB-YP759244EYA
CSB-EP759244EYA
CSB-BP759244EYA
CSB-MP759244EYA
CSB-EP759244EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Adenylosuccinate synthetase 2 (purA2) CSB-YP759245PIJ
CSB-EP759245PIJ
CSB-BP759245PIJ
CSB-MP759245PIJ
CSB-EP759245PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP759246PIJ
CSB-EP759246PIJ
CSB-BP759246PIJ
CSB-MP759246PIJ
CSB-EP759246PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Acetyl-coenzyme A synthetase (acs), partial CSB-YP759247PIJ
CSB-EP759247PIJ
CSB-BP759247PIJ
CSB-MP759247PIJ
CSB-EP759247PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP759248GCI
CSB-EP759248GCI
CSB-BP759248GCI
CSB-MP759248GCI
CSB-EP759248GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP759249CKA
CSB-EP759249CKA
CSB-BP759249CKA
CSB-MP759249CKA
CSB-EP759249CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP759250VCQ
CSB-EP759250VCQ
CSB-BP759250VCQ
CSB-MP759250VCQ
CSB-EP759250VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Tyrosine--tRNA ligase 1 (tyrS1) CSB-YP759251VCQ
CSB-EP759251VCQ
CSB-BP759251VCQ
CSB-MP759251VCQ
CSB-EP759251VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus N-acetylmannosamine kinase (nanK) CSB-YP759252VCQ
CSB-EP759252VCQ
CSB-BP759252VCQ
CSB-MP759252VCQ
CSB-EP759252VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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