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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Danio rerio Eukaryotic translation initiation factor 3 subunit J-A (eif3ja) CSB-YP759673DIL
CSB-EP759673DIL
CSB-BP759673DIL
CSB-MP759673DIL
CSB-EP759673DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Multivesicular body subunit 12A (fam125a) CSB-YP759674DIL
CSB-EP759674DIL
CSB-BP759674DIL
CSB-MP759674DIL
CSB-EP759674DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein kinase C beta type (prkcbb), partial CSB-YP759675DIL
CSB-EP759675DIL
CSB-BP759675DIL
CSB-MP759675DIL
CSB-EP759675DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Monoacylglycerol lipase abhd6-B (abhd6-b), partial CSB-YP759676XBE
CSB-EP759676XBE
CSB-BP759676XBE
CSB-MP759676XBE
CSB-EP759676XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein transport protein Sec31A (sec31a), partial CSB-YP759677DIL
CSB-EP759677DIL
CSB-BP759677DIL
CSB-MP759677DIL
CSB-EP759677DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Chitinase domain-containing protein 1 (chid1) CSB-YP759678DIL
CSB-EP759678DIL
CSB-BP759678DIL
CSB-MP759678DIL
CSB-EP759678DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Probable lysosomal cobalamin transporter (lmbrd1), partial CSB-YP759679XBE
CSB-EP759679XBE
CSB-BP759679XBE
CSB-MP759679XBE
CSB-EP759679XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Ragulator complex protein LAMTOR1 (lamtor1) CSB-YP759680XBE
CSB-EP759680XBE
CSB-BP759680XBE
CSB-MP759680XBE
CSB-EP759680XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis F-box/LRR-repeat protein 15 (fbxl15) CSB-YP759681XBE
CSB-EP759681XBE
CSB-BP759681XBE
CSB-MP759681XBE
CSB-EP759681XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloydius ussuriensis Zinc metalloproteinase/disintegrin ussurin CSB-YP759682GGS
CSB-EP759682GGS
CSB-BP759682GGS
CSB-MP759682GGS
CSB-EP759682GGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein transport protein Sec23A (sec23a), partial CSB-YP759683DIL
CSB-EP759683DIL
CSB-BP759683DIL
CSB-MP759683DIL
CSB-EP759683DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio UPF0690 protein C1orf52 homolog (zgc:65873) CSB-YP759684DIL
CSB-EP759684DIL
CSB-BP759684DIL
CSB-MP759684DIL
CSB-EP759684DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Surfeit locus protein 4 (surf4), partial CSB-YP759685DIL1
CSB-EP759685DIL1
CSB-BP759685DIL1
CSB-MP759685DIL1
CSB-EP759685DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Rab11 family-interacting protein 3 (rab11fip3), partial CSB-YP759686DIL
CSB-EP759686DIL
CSB-BP759686DIL
CSB-MP759686DIL
CSB-EP759686DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrovipera lebetina Coagulation factor X-activating enzyme heavy chain CSB-YP759687MPJ
CSB-EP759687MPJ
CSB-BP759687MPJ
CSB-MP759687MPJ
CSB-EP759687MPJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Histone chaperone asf1a-B (asf1ab) CSB-YP759688XBE
CSB-EP759688XBE
CSB-BP759688XBE
CSB-MP759688XBE
CSB-EP759688XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis NudC domain-containing protein 1 (nudcd1), partial CSB-YP759689XBE
CSB-EP759689XBE
CSB-BP759689XBE
CSB-MP759689XBE
CSB-EP759689XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Sodium/bile acid cotransporter 7-A (slc10a7-a), partial CSB-YP759690XBE
CSB-EP759690XBE
CSB-BP759690XBE
CSB-MP759690XBE
CSB-EP759690XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Methionine--tRNA ligase, mitochondrial (mars2), partial CSB-YP759691XBE
CSB-EP759691XBE
CSB-BP759691XBE
CSB-MP759691XBE
CSB-EP759691XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opsanus tau Acid-sensing ion channel 1 (asic1), partial CSB-YP759692OBX
CSB-EP759692OBX
CSB-BP759692OBX
CSB-MP759692OBX
CSB-EP759692OBX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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