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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Neurospora crassa Crossover junction endonuclease mus-81 (mus-81), partial CSB-YP759653NHA
CSB-EP759653NHA
CSB-BP759653NHA
CSB-MP759653NHA
CSB-EP759653NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Probable lysosomal cobalamin transporter (NCU02100), partial CSB-YP759654NHA
CSB-EP759654NHA
CSB-BP759654NHA
CSB-MP759654NHA
CSB-EP759654NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Autophagy-related protein 3 (atg-3) CSB-YP759655NHA
CSB-EP759655NHA
CSB-BP759655NHA
CSB-MP759655NHA
CSB-EP759655NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Presequence translocated-associated motor subunit pam-17, mitochondrial (pam-17), partial CSB-YP759656NHA1
CSB-EP759656NHA1
CSB-BP759656NHA1
CSB-MP759656NHA1
CSB-EP759656NHA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Molybdopterin synthase catalytic subunit (mocs-2) CSB-YP759657NHA
CSB-EP759657NHA
CSB-BP759657NHA
CSB-MP759657NHA
CSB-EP759657NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Lipoyl synthase, mitochondrial (NCU00565) CSB-YP759658NHA
CSB-EP759658NHA
CSB-BP759658NHA
CSB-MP759658NHA
CSB-EP759658NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Pre-rRNA-processing protein ipi-1 (ipi-1) CSB-YP759659NHA
CSB-EP759659NHA
CSB-BP759659NHA
CSB-MP759659NHA
CSB-EP759659NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Multiple RNA-binding domain-containing protein 1 (mrd-1), partial CSB-YP759660NHA
CSB-EP759660NHA
CSB-BP759660NHA
CSB-MP759660NHA
CSB-EP759660NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa NAD (P)H-hydrate epimerase (NCU08070) CSB-YP759661NHA
CSB-EP759661NHA
CSB-BP759661NHA
CSB-MP759661NHA
CSB-EP759661NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Putative D-arabinono-1,4-lactone oxidase (alo-1), partial CSB-YP759662NHA
CSB-EP759662NHA
CSB-BP759662NHA
CSB-MP759662NHA
CSB-EP759662NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Sorting nexin-3 (snx-3) CSB-YP759663NHA
CSB-EP759663NHA
CSB-BP759663NHA
CSB-MP759663NHA
CSB-EP759663NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Pre-mRNA-splicing factor isy-1 (isy-1) CSB-YP759664NHA
CSB-EP759664NHA
CSB-BP759664NHA
CSB-MP759664NHA
CSB-EP759664NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Acetylornithine/acetyl-lysine aminotransferase (argD) CSB-YP759665FPM
CSB-EP759665FPM
CSB-BP759665FPM
CSB-MP759665FPM
CSB-EP759665FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP759666TNT
CSB-EP759666TNT
CSB-BP759666TNT
CSB-MP759666TNT
CSB-EP759666TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cobalt-containing nitrile hydratase subunit alpha CSB-YP759667PHAR
CSB-EP759667PHAR
CSB-BP759667PHAR
CSB-MP759667PHAR
CSB-EP759667PHAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gracilaria chilensis R-phycoerythrin alpha chain (rpeA) CSB-YP759668GCAA
CSB-EP759668GCAA
CSB-BP759668GCAA
CSB-MP759668GCAA
CSB-EP759668GCAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian leukosis virus Pol polyprotein (pol), partial CSB-YP759669ASM
CSB-EP759669ASM
CSB-BP759669ASM
CSB-MP759669ASM
CSB-EP759669ASM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Ubiquitin-like protein 5 (ubl5) CSB-YP759670DIL
CSB-EP759670DIL
CSB-BP759670DIL
CSB-MP759670DIL
CSB-EP759670DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio U4/U6 small nuclear ribonucleoprotein Prp31 (prpf31), partial CSB-YP759671DIL
CSB-EP759671DIL
CSB-BP759671DIL
CSB-MP759671DIL
CSB-EP759671DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio NEDD8-activating enzyme E1 regulatory subunit (nae1), partial CSB-YP759672DIL
CSB-EP759672DIL
CSB-BP759672DIL
CSB-MP759672DIL
CSB-EP759672DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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