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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Neurospora crassa Mitochondrial thiamine pyrophosphate carrier 1 (tpc-1), partial CSB-YP759633NHA1
CSB-EP759633NHA1
CSB-BP759633NHA1
CSB-MP759633NHA1
CSB-EP759633NHA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Protein dml-1 (dml-1), partial CSB-YP759634NHA
CSB-EP759634NHA
CSB-BP759634NHA
CSB-MP759634NHA
CSB-EP759634NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa DNA replication regulator sld-2 (sld-2), partial CSB-YP759635NHA
CSB-EP759635NHA
CSB-BP759635NHA
CSB-MP759635NHA
CSB-EP759635NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa WD repeat-containing protein jip-5 (jip-5) CSB-YP759636NHA
CSB-EP759636NHA
CSB-BP759636NHA
CSB-MP759636NHA
CSB-EP759636NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa GPI mannosyltransferase 1 (gpi-14), partial CSB-YP759637NHA
CSB-EP759637NHA
CSB-BP759637NHA
CSB-MP759637NHA
CSB-EP759637NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Nucleolar protein 9 (nop9), partial CSB-YP759638NHA
CSB-EP759638NHA
CSB-BP759638NHA
CSB-MP759638NHA
CSB-EP759638NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Serine/threonine-protein phosphatase 2A activator 1 (rrd-1), partial CSB-YP759639NHA
CSB-EP759639NHA
CSB-BP759639NHA
CSB-MP759639NHA
CSB-EP759639NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa 2',3'-cyclic-nucleotide 3'-phosphodiesterase (cpd-1) CSB-YP759640NHA
CSB-EP759640NHA
CSB-BP759640NHA
CSB-MP759640NHA
CSB-EP759640NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Mitochondrial presequence protease (cym-1), partial CSB-YP759641NHA
CSB-EP759641NHA
CSB-BP759641NHA
CSB-MP759641NHA
CSB-EP759641NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa 37S ribosomal protein S9, mitochondrial (82C3.030, NCU03827), partial CSB-YP759642NHA
CSB-EP759642NHA
CSB-BP759642NHA
CSB-MP759642NHA
CSB-EP759642NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Dicer-like protein 1 (dcl-1), partial CSB-YP759643NHA
CSB-EP759643NHA
CSB-BP759643NHA
CSB-MP759643NHA
CSB-EP759643NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Stress response protein nst-1 (nst-1), partial CSB-YP759644NHA
CSB-EP759644NHA
CSB-BP759644NHA
CSB-MP759644NHA
CSB-EP759644NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Histone acetyltransferase esa-1 (esa-1), partial CSB-YP759645NHA
CSB-EP759645NHA
CSB-BP759645NHA
CSB-MP759645NHA
CSB-EP759645NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Vacuolar membrane-associated protein iml-1 (iml-1), partial CSB-YP759646NHA
CSB-EP759646NHA
CSB-BP759646NHA
CSB-MP759646NHA
CSB-EP759646NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Exoglucanase 1 (cbh-1), partial CSB-YP759647NHA
CSB-EP759647NHA
CSB-BP759647NHA
CSB-MP759647NHA
CSB-EP759647NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Pre-mRNA-splicing factor cef-1 (cef-1), partial CSB-YP759648NHA
CSB-EP759648NHA
CSB-BP759648NHA
CSB-MP759648NHA
CSB-EP759648NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa AP-2 complex subunit sigma (aps-2) CSB-YP759649NHA
CSB-EP759649NHA
CSB-BP759649NHA
CSB-MP759649NHA
CSB-EP759649NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Sorting nexin mvp-1 (mvp-1), partial CSB-YP759650NHA
CSB-EP759650NHA
CSB-BP759650NHA
CSB-MP759650NHA
CSB-EP759650NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Enolase-phosphatase E1 (utr-4) CSB-YP759651NHA
CSB-EP759651NHA
CSB-BP759651NHA
CSB-MP759651NHA
CSB-EP759651NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Dicer-like protein 2 (dcl-2), partial CSB-YP759652NHA
CSB-EP759652NHA
CSB-BP759652NHA
CSB-MP759652NHA
CSB-EP759652NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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