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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Prochlorococcus marinus Lysine--tRNA ligase (lysS), partial CSB-YP759793EYO
CSB-EP759793EYO
CSB-BP759793EYO
CSB-MP759793EYO
CSB-EP759793EYO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP759794SSL
CSB-EP759794SSL
CSB-BP759794SSL
CSB-MP759794SSL
CSB-EP759794SSL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Biosynthetic arginine decarboxylase (speA), partial CSB-YP759795SSL
CSB-EP759795SSL
CSB-BP759795SSL
CSB-MP759795SSL
CSB-EP759795SSL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L10 (rplJ) CSB-YP759796SSL
CSB-EP759796SSL
CSB-BP759796SSL
CSB-MP759796SSL
CSB-EP759796SSL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Probable rRNA maturation factor (SYNW2313) CSB-YP759797SSL
CSB-EP759797SSL
CSB-BP759797SSL
CSB-MP759797SSL
CSB-EP759797SSL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. NAD (P)H-quinone oxidoreductase subunit 1 (ndhA), partial CSB-YP759798SSL
CSB-EP759798SSL
CSB-BP759798SSL
CSB-MP759798SSL
CSB-EP759798SSL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. NAD (P)H-quinone oxidoreductase subunit O (ndhO) CSB-YP759799SSL
CSB-EP759799SSL
CSB-BP759799SSL
CSB-MP759799SSL
CSB-EP759799SSL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. LL-diaminopimelate aminotransferase (dapL) CSB-YP759800SSL
CSB-EP759800SSL
CSB-BP759800SSL
CSB-MP759800SSL
CSB-EP759800SSL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Lipoyl synthase 1 (lipA1) CSB-YP759801SSL
CSB-EP759801SSL
CSB-BP759801SSL
CSB-MP759801SSL
CSB-EP759801SSL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L17 (rplQ) CSB-YP759802SSL
CSB-EP759802SSL
CSB-BP759802SSL
CSB-MP759802SSL
CSB-EP759802SSL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L18 (rplR) CSB-YP759803SSL
CSB-EP759803SSL
CSB-BP759803SSL
CSB-MP759803SSL
CSB-EP759803SSL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L16 (rplP) CSB-YP759804SSL
CSB-EP759804SSL
CSB-BP759804SSL
CSB-MP759804SSL
CSB-EP759804SSL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L4 (rplD) CSB-YP759805SSL
CSB-EP759805SSL
CSB-BP759805SSL
CSB-MP759805SSL
CSB-EP759805SSL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP759806SSL
CSB-EP759806SSL
CSB-BP759806SSL
CSB-MP759806SSL
CSB-EP759806SSL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Cell division topological specificity factor (minE) CSB-YP759807SSL
CSB-EP759807SSL
CSB-BP759807SSL
CSB-MP759807SSL
CSB-EP759807SSL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. GTPase obg (obg) CSB-YP759808SSL
CSB-EP759808SSL
CSB-BP759808SSL
CSB-MP759808SSL
CSB-EP759808SSL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribonuclease P protein component (rnpA) CSB-YP759809SSL
CSB-EP759809SSL
CSB-BP759809SSL
CSB-MP759809SSL
CSB-EP759809SSL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Cytochrome b6-f complex iron-sulfur subunit (petC), partial CSB-YP759810SSL1
CSB-EP759810SSL1
CSB-BP759810SSL1
CSB-MP759810SSL1
CSB-EP759810SSL1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Acetylglutamate kinase (argB) CSB-YP759811SSL
CSB-EP759811SSL
CSB-BP759811SSL
CSB-MP759811SSL
CSB-EP759811SSL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP759812SSL
CSB-EP759812SSL
CSB-BP759812SSL
CSB-MP759812SSL
CSB-EP759812SSL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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