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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus UvrABC system protein C (uvrC), partial CSB-YP759993EYP
CSB-EP759993EYP
CSB-BP759993EYP
CSB-MP759993EYP
CSB-EP759993EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP759994EYP
CSB-EP759994EYP
CSB-BP759994EYP
CSB-MP759994EYP
CSB-EP759994EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) CSB-YP759995EYP
CSB-EP759995EYP
CSB-BP759995EYP
CSB-MP759995EYP
CSB-EP759995EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP759996EYP
CSB-EP759996EYP
CSB-BP759996EYP
CSB-MP759996EYP
CSB-EP759996EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Histidine--tRNA ligase (hisS) CSB-YP759997EYP
CSB-EP759997EYP
CSB-BP759997EYP
CSB-MP759997EYP
CSB-EP759997EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP759998EYP
CSB-EP759998EYP
CSB-BP759998EYP
CSB-MP759998EYP
CSB-EP759998EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Tryptophan synthase alpha chain (trpA) CSB-YP759999EYP
CSB-EP759999EYP
CSB-BP759999EYP
CSB-MP759999EYP
CSB-EP759999EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Light-independent protochlorophyllide reductase subunit B (chlB), partial CSB-YP760000EYP
CSB-EP760000EYP
CSB-BP760000EYP
CSB-MP760000EYP
CSB-EP760000EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Porphobilinogen deaminase (hemC) CSB-YP760001EYP
CSB-EP760001EYP
CSB-BP760001EYP
CSB-MP760001EYP
CSB-EP760001EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Guanylate kinase (gmk) CSB-YP760002EYP
CSB-EP760002EYP
CSB-BP760002EYP
CSB-MP760002EYP
CSB-EP760002EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Elongation factor 4 (lepA), partial CSB-YP760003EYP
CSB-EP760003EYP
CSB-BP760003EYP
CSB-MP760003EYP
CSB-EP760003EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome b6-f complex subunit 4 (petD), partial CSB-YP760004EYP1
CSB-EP760004EYP1
CSB-BP760004EYP1
CSB-MP760004EYP1
CSB-EP760004EYP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus S-adenosylmethionine synthase (metK) CSB-YP760005EYP
CSB-EP760005EYP
CSB-BP760005EYP
CSB-MP760005EYP
CSB-EP760005EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit K (ndhK) CSB-YP760006EYP
CSB-EP760006EYP
CSB-BP760006EYP
CSB-MP760006EYP
CSB-EP760006EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP760007EYP
CSB-EP760007EYP
CSB-BP760007EYP
CSB-MP760007EYP
CSB-EP760007EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Phosphoglucosamine mutase (glmM) CSB-YP760008EYP
CSB-EP760008EYP
CSB-BP760008EYP
CSB-MP760008EYP
CSB-EP760008EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L1 (rplA) CSB-YP760009EYP
CSB-EP760009EYP
CSB-BP760009EYP
CSB-MP760009EYP
CSB-EP760009EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Arginine--tRNA ligase (argS), partial CSB-YP760010EYP
CSB-EP760010EYP
CSB-BP760010EYP
CSB-MP760010EYP
CSB-EP760010EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit I (ndhI) CSB-YP760011EYP
CSB-EP760011EYP
CSB-BP760011EYP
CSB-MP760011EYP
CSB-EP760011EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit O (ndhO) CSB-YP760012EYP
CSB-EP760012EYP
CSB-BP760012EYP
CSB-MP760012EYP
CSB-EP760012EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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