Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Synechococcus sp. 30S ribosomal protein S4 (rpsD) CSB-YP759813SSL
CSB-EP759813SSL
CSB-BP759813SSL
CSB-MP759813SSL
CSB-EP759813SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. D-alanine--D-alanine ligase (ddl) CSB-YP759814SSL
CSB-EP759814SSL
CSB-BP759814SSL
CSB-MP759814SSL
CSB-EP759814SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Chaperone protein ClpB 1 (clpB1), partial CSB-YP759815SSL
CSB-EP759815SSL
CSB-BP759815SSL
CSB-MP759815SSL
CSB-EP759815SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribosomal protein S6 modification protein (rimK) CSB-YP759816SSL
CSB-EP759816SSL
CSB-BP759816SSL
CSB-MP759816SSL
CSB-EP759816SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP759817SSL
CSB-EP759817SSL
CSB-BP759817SSL
CSB-MP759817SSL
CSB-EP759817SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. UvrABC system protein C (uvrC), partial CSB-YP759818SSL
CSB-EP759818SSL
CSB-BP759818SSL
CSB-MP759818SSL
CSB-EP759818SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase (acsF) CSB-YP759819SSL
CSB-EP759819SSL
CSB-BP759819SSL
CSB-MP759819SSL
CSB-EP759819SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Elongation factor Ts (tsf) CSB-YP759820SSL
CSB-EP759820SSL
CSB-BP759820SSL
CSB-MP759820SSL
CSB-EP759820SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Bifunctional protein GlmU (glmU) CSB-YP759821SSL
CSB-EP759821SSL
CSB-BP759821SSL
CSB-MP759821SSL
CSB-EP759821SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP759822SSL
CSB-EP759822SSL
CSB-BP759822SSL
CSB-MP759822SSL
CSB-EP759822SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA (cpdA) CSB-YP759823SSL
CSB-EP759823SSL
CSB-BP759823SSL
CSB-MP759823SSL
CSB-EP759823SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 3-isopropylmalate dehydrogenase (leuB) CSB-YP759824SSL
CSB-EP759824SSL
CSB-BP759824SSL
CSB-MP759824SSL
CSB-EP759824SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Glutamate 5-kinase (proB) CSB-YP759825SSL
CSB-EP759825SSL
CSB-BP759825SSL
CSB-MP759825SSL
CSB-EP759825SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Octanoyltransferase (lipB) CSB-YP759826SSL
CSB-EP759826SSL
CSB-BP759826SSL
CSB-MP759826SSL
CSB-EP759826SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. GTPase Der (der) CSB-YP759827SSL
CSB-EP759827SSL
CSB-BP759827SSL
CSB-MP759827SSL
CSB-EP759827SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Fumarate hydratase class II (fumC) CSB-YP759828SSL
CSB-EP759828SSL
CSB-BP759828SSL
CSB-MP759828SSL
CSB-EP759828SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP759829SSL
CSB-EP759829SSL
CSB-BP759829SSL
CSB-MP759829SSL
CSB-EP759829SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Histidinol dehydrogenase (hisD) CSB-YP759830SSL
CSB-EP759830SSL
CSB-BP759830SSL
CSB-MP759830SSL
CSB-EP759830SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase (fabZ) CSB-YP759831SSL
CSB-EP759831SSL
CSB-BP759831SSL
CSB-MP759831SSL
CSB-EP759831SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Probable transcriptional regulatory protein SYNW0543 (SYNW0543) CSB-YP759832SSL
CSB-EP759832SSL
CSB-BP759832SSL
CSB-MP759832SSL
CSB-EP759832SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>