Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Oryza sativa subsp. japonica Probable 6-phosphogluconolactonase 3, chloroplastic (Os08g0547100, LOC_Os08g43370) CSB-YP761615OFG
CSB-EP761615OFG
CSB-BP761615OFG
CSB-MP761615OFG
CSB-EP761615OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Endoglucanase 20 (GLU15) CSB-YP761616OFG
CSB-EP761616OFG
CSB-BP761616OFG
CSB-MP761616OFG
CSB-EP761616OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable protein NAP1 (NAP1), partial CSB-YP761617OFG
CSB-EP761617OFG
CSB-BP761617OFG
CSB-MP761617OFG
CSB-EP761617OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Germin-like protein 8-12 (Os08g0231400, LOC_Os08g13440) CSB-YP761618OFG
CSB-EP761618OFG
CSB-BP761618OFG
CSB-MP761618OFG
CSB-EP761618OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Thioredoxin reductase NTRB (NTRB) CSB-YP761619OFG
CSB-EP761619OFG
CSB-BP761619OFG
CSB-MP761619OFG
CSB-EP761619OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Autophagy protein 5 (ATG5) CSB-YP761620OFG
CSB-EP761620OFG
CSB-BP761620OFG
CSB-MP761620OFG
CSB-EP761620OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Elongation factor Ts, mitochondrial (EFTS) CSB-YP761621OFG
CSB-EP761621OFG
CSB-BP761621OFG
CSB-MP761621OFG
CSB-EP761621OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable histone H2A.1 (Os07g0545300, LOC_Os07g36130) CSB-YP761622OFG
CSB-EP761622OFG
CSB-BP761622OFG
CSB-MP761622OFG
CSB-EP761622OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Sialic acid-binding Ig-like lectin 15 (SIGLEC15), partial CSB-YP761623HU
CSB-BP761623HU
CSB-EP761623HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human ADAMTS-like protein 5 (ADAMTSL5) CSB-YP761624HU
CSB-BP761624HU
CSB-MP761624HU
CSB-EP761624HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Serine/threonine-protein kinase LMTK1 (AATK), partial CSB-YP761625HU
CSB-EP761625HU
CSB-BP761625HU
CSB-MP761625HU
CSB-EP761625HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human E3 ubiquitin-protein ligase RNF19B (RNF19B), partial CSB-YP761626HU1
CSB-EP761626HU1
CSB-BP761626HU1
CSB-MP761626HU1
CSB-EP761626HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Metastasis-associated in colon cancer protein 1 (MACC1), partial CSB-YP761627HU
CSB-EP761627HU
CSB-BP761627HU
CSB-MP761627HU
CSB-EP761627HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative maltase-glucoamylase-like protein FLJ16351 CSB-YP761628HU
CSB-EP761628HU
CSB-BP761628HU
CSB-MP761628HU
CSB-EP761628HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Homeobox protein DBX2 (DBX2) CSB-YP761629HU
CSB-EP761629HU
CSB-BP761629HU
CSB-MP761629HU
CSB-EP761629HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human TRAF-interacting protein with FHA domain-containing protein B (TIFAB) CSB-YP761630HU
CSB-EP761630HU
CSB-BP761630HU
CSB-MP761630HU
CSB-EP761630HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative uncharacterized protein C13orf35 (C13orf35) CSB-YP761631HU
CSB-EP761631HU
CSB-BP761631HU
CSB-MP761631HU
CSB-EP761631HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Myotubularin-related protein 5 (Sbf1), partial CSB-YP761632MO
CSB-EP761632MO
CSB-BP761632MO
CSB-MP761632MO
CSB-EP761632MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Membrane-associated phosphatidylinositol transfer protein 2 (Pitpnm2), partial CSB-YP761633MO
CSB-EP761633MO
CSB-BP761633MO
CSB-MP761633MO
CSB-EP761633MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse DNA helicase INO80 (Ino80), partial CSB-YP761634MO
CSB-EP761634MO
CSB-BP761634MO
CSB-MP761634MO
CSB-EP761634MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>