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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Trehalose-phosphate synthase (otsA) CSB-YP762076MLA
CSB-EP762076MLA
CSB-BP762076MLA
CSB-MP762076MLA
CSB-EP762076MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium paratuberculosis DNA integrity scanning protein DisA (disA) CSB-YP762077MLA
CSB-BP762077MLA
CSB-MP762077MLA
CSB-EP762077MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0301 protein MAP_0045 (MAP_0045) CSB-YP762078MLA
CSB-EP762078MLA
CSB-BP762078MLA
CSB-MP762078MLA
CSB-EP762078MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serine--tRNA ligase (serS) CSB-YP762079MLA
CSB-EP762079MLA
CSB-BP762079MLA
CSB-MP762079MLA
CSB-EP762079MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP762080GBK
CSB-EP762080GBK
CSB-BP762080GBK
CSB-MP762080GBK
CSB-EP762080GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Phosphoenolpyruvate carboxykinase [GTP] (pckG), partial CSB-YP762081GBK
CSB-EP762081GBK
CSB-BP762081GBK
CSB-MP762081GBK
CSB-EP762081GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 60 kDa chaperonin (groL), partial CSB-YP762082GBK
CSB-EP762082GBK
CSB-BP762082GBK
CSB-MP762082GBK
CSB-EP762082GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens UvrABC system protein B (uvrB), partial CSB-YP762083GBK
CSB-EP762083GBK
CSB-BP762083GBK
CSB-MP762083GBK
CSB-EP762083GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Glutamate 5-kinase (proB) CSB-YP762084GBK
CSB-EP762084GBK
CSB-BP762084GBK
CSB-MP762084GBK
CSB-EP762084GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP762085GBK
CSB-EP762085GBK
CSB-BP762085GBK
CSB-MP762085GBK
CSB-EP762085GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP762086GBK
CSB-EP762086GBK
CSB-BP762086GBK
CSB-MP762086GBK
CSB-EP762086GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Lipid II flippase FtsW (ftsW), partial CSB-YP762087GBK
CSB-EP762087GBK
CSB-BP762087GBK
CSB-MP762087GBK
CSB-EP762087GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Probable GTP-binding protein EngB (engB) CSB-YP762088GBK
CSB-EP762088GBK
CSB-BP762088GBK
CSB-MP762088GBK
CSB-EP762088GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP762089GBK
CSB-EP762089GBK
CSB-BP762089GBK
CSB-MP762089GBK
CSB-EP762089GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 30S ribosomal protein S10 (rpsJ) CSB-YP762090GBK
CSB-EP762090GBK
CSB-BP762090GBK
CSB-MP762090GBK
CSB-EP762090GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 50S ribosomal protein L14 (rplN) CSB-YP762091GBK
CSB-EP762091GBK
CSB-BP762091GBK
CSB-MP762091GBK
CSB-EP762091GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Adenylate kinase (adk) CSB-YP762092GBK
CSB-EP762092GBK
CSB-BP762092GBK
CSB-MP762092GBK
CSB-EP762092GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Acetate kinase (ackA) CSB-YP762093GBK
CSB-EP762093GBK
CSB-BP762093GBK
CSB-MP762093GBK
CSB-EP762093GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP762094GBK
CSB-EP762094GBK
CSB-BP762094GBK
CSB-MP762094GBK
CSB-EP762094GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Octanoyltransferase (lipB) CSB-YP762095GBK
CSB-EP762095GBK
CSB-BP762095GBK
CSB-MP762095GBK
CSB-EP762095GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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