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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP761936BQN
CSB-EP761936BQN
CSB-BP761936BQN
CSB-MP761936BQN
CSB-EP761936BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Aspartate--tRNA ligase (aspS), partial CSB-YP761937BQN
CSB-EP761937BQN
CSB-BP761937BQN
CSB-MP761937BQN
CSB-EP761937BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Uridine kinase (udk) CSB-YP761938BQN
CSB-EP761938BQN
CSB-BP761938BQN
CSB-MP761938BQN
CSB-EP761938BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Shikimate dehydrogenase (aroE) CSB-YP761939BQN
CSB-EP761939BQN
CSB-BP761939BQN
CSB-MP761939BQN
CSB-EP761939BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Heat-inducible transcription repressor HrcA (hrcA) CSB-YP761940BQN
CSB-EP761940BQN
CSB-BP761940BQN
CSB-MP761940BQN
CSB-EP761940BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP761941BQN
CSB-EP761941BQN
CSB-BP761941BQN
CSB-MP761941BQN
CSB-EP761941BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP761942BQN
CSB-EP761942BQN
CSB-BP761942BQN
CSB-MP761942BQN
CSB-EP761942BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP761943BQN
CSB-EP761943BQN
CSB-BP761943BQN
CSB-MP761943BQN
CSB-EP761943BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP761944BQN
CSB-EP761944BQN
CSB-BP761944BQN
CSB-MP761944BQN
CSB-EP761944BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Heme A synthase (ctaA), partial CSB-YP761945BQN
CSB-EP761945BQN
CSB-BP761945BQN
CSB-MP761945BQN
CSB-EP761945BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Isoleucine--tRNA ligase 1 (ileS1), partial CSB-YP761946BQN
CSB-EP761946BQN
CSB-BP761946BQN
CSB-MP761946BQN
CSB-EP761946BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Dihydroorotate dehydrogenase B (NAD (+)), electron transfer subunit (pyrK) CSB-YP761947BQN
CSB-EP761947BQN
CSB-BP761947BQN
CSB-MP761947BQN
CSB-EP761947BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribonuclease HII (rnhB) CSB-YP761948BQN
CSB-EP761948BQN
CSB-BP761948BQN
CSB-MP761948BQN
CSB-EP761948BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Translation initiation factor IF-2 (infB), partial CSB-YP761949BQN
CSB-EP761949BQN
CSB-BP761949BQN
CSB-MP761949BQN
CSB-EP761949BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus tRNA pseudouridine synthase B (truB) CSB-YP761950BQN
CSB-EP761950BQN
CSB-BP761950BQN
CSB-MP761950BQN
CSB-EP761950BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Tat CSB-YP761951HDAK
CSB-EP761951HDAK
CSB-BP761951HDAK
CSB-MP761951HDAK
CSB-EP761951HDAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Holin-like protein CidA (cidA), partial CSB-YP761952BQN1
CSB-EP761952BQN1
CSB-BP761952BQN1
CSB-MP761952BQN1
CSB-EP761952BQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Urease subunit beta (ureB) CSB-YP761953BQN
CSB-EP761953BQN
CSB-BP761953BQN
CSB-MP761953BQN
CSB-EP761953BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glycerol-3-phosphate acyltransferase 2 (plsY2), partial CSB-YP761954BQN1
CSB-EP761954BQN1
CSB-BP761954BQN1
CSB-MP761954BQN1
CSB-EP761954BQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NAD-dependent protein deacetylase (cobB) CSB-YP761955BQN
CSB-EP761955BQN
CSB-BP761955BQN
CSB-MP761955BQN
CSB-EP761955BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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