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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechococcus sp. Ferrochelatase (hemH) CSB-YP008579SVC
CSB-EP008579SVC
CSB-BP008579SVC
CSB-MP008579SVC
CSB-EP008579SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Thymidylate kinase (tmk) CSB-YP007226SVB
CSB-EP007226SVB
CSB-BP007226SVB
CSB-MP007226SVB
CSB-EP007226SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ferrochelatase (hemH) CSB-YP008579SVB
CSB-EP008579SVB
CSB-BP008579SVB
CSB-MP008579SVB
CSB-EP008579SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Methionyl-tRNA formyltransferase (fmt) CSB-YP015150SVB
CSB-EP015150SVB
CSB-BP015150SVB
CSB-MP015150SVB
CSB-EP015150SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Elongation factor Tu (tuf) CSB-YP025342SVB
CSB-EP025342SVB
CSB-BP025342SVB
CSB-MP025342SVB
CSB-EP025342SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Porphobilinogen deaminase (hemC) CSB-YP010524CWV
CSB-EP010524CWV
CSB-BP010524CWV
CSB-MP010524CWV
CSB-EP010524CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Serine hydroxymethyltransferase (glyA) CSB-YP021274CWV
CSB-EP021274CWV
CSB-BP021274CWV
CSB-MP021274CWV
CSB-EP021274CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Phosphoserine aminotransferase (serC) CSB-YP018838LLJ
CSB-EP018838LLJ
CSB-BP018838LLJ
CSB-MP018838LLJ
CSB-EP018838LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Ribonuclease 3 (rnc) CSB-YP019809LLJ
CSB-EP019809LLJ
CSB-BP019809LLJ
CSB-MP019809LLJ
CSB-EP019809LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila GTPase Era (era) CSB-YP007759TKF
CSB-EP007759TKF
CSB-BP007759TKF
CSB-MP007759TKF
CSB-EP007759TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582TKF
CSB-EP009582TKF
CSB-BP009582TKF
CSB-MP009582TKF
CSB-EP009582TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Elongation factor Ts (tsf) CSB-YP025124TKF
CSB-EP025124TKF
CSB-BP025124TKF
CSB-MP025124TKF
CSB-EP025124TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Thymidylate kinase (tmk) CSB-YP007226TKF
CSB-EP007226TKF
CSB-BP007226TKF
CSB-MP007226TKF
CSB-EP007226TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Acyl carrier protein (acpP) CSB-YP015636SUJ
CSB-EP015636SUJ
CSB-BP015636SUJ
CSB-MP015636SUJ
CSB-EP015636SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae Serine hydroxymethyltransferase (glyA) CSB-YP021274MWC
CSB-EP021274MWC
CSB-BP021274MWC
CSB-MP021274MWC
CSB-EP021274MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium vivax Alpha-tubulin N-acetyltransferase (PVX_099660) CSB-YP004059EWW
CSB-EP004059EWW
CSB-BP004059EWW
CSB-MP004059EWW
CSB-EP004059EWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri ATP synthase subunit beta (atpD) CSB-YP002350CWX
CSB-EP002350CWX
CSB-BP002350CWX
CSB-MP002350CWX
CSB-EP002350CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Elongation factor G (fusA), partial CSB-YP009373CWX
CSB-EP009373CWX
CSB-BP009373CWX
CSB-MP009373CWX
CSB-EP009373CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio GTPase-activating Rap/Ran-GAP domain-like protein 3 (garnl3), partial CSB-YP009260DIL
CSB-EP009260DIL
CSB-BP009260DIL
CSB-MP009260DIL
CSB-EP009260DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Protein TSC21 (TSC21) CSB-YP003852BO
CSB-EP003852BO
CSB-BP003852BO
CSB-MP003852BO
CSB-EP003852BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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