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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Synechococcus sp. Ribonuclease Z (rnz) CSB-YP763280SSL
CSB-EP763280SSL
CSB-BP763280SSL
CSB-MP763280SSL
CSB-EP763280SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. ATP synthase subunit alpha (atpA), partial CSB-YP763281SSL
CSB-EP763281SSL
CSB-BP763281SSL
CSB-MP763281SSL
CSB-EP763281SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Peptide deformylase (def) CSB-YP763282SSL
CSB-EP763282SSL
CSB-BP763282SSL
CSB-MP763282SSL
CSB-EP763282SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Peptidyl-tRNA hydrolase (pth) CSB-YP763283SSL
CSB-EP763283SSL
CSB-BP763283SSL
CSB-MP763283SSL
CSB-EP763283SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP763284SSL
CSB-EP763284SSL
CSB-BP763284SSL
CSB-MP763284SSL
CSB-EP763284SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ycf48-like protein (SYNW0205) CSB-YP763285SSL
CSB-EP763285SSL
CSB-BP763285SSL
CSB-MP763285SSL
CSB-EP763285SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribosome maturation factor RimM (rimM) CSB-YP763286SSL
CSB-EP763286SSL
CSB-BP763286SSL
CSB-MP763286SSL
CSB-EP763286SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Protein CrcB homolog 2 (crcB2), partial CSB-YP763287SSL1
CSB-EP763287SSL1
CSB-BP763287SSL1
CSB-MP763287SSL1
CSB-EP763287SSL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. tRNA (Ile)-lysidine synthase (tilS) CSB-YP763288SSL
CSB-EP763288SSL
CSB-BP763288SSL
CSB-MP763288SSL
CSB-EP763288SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Thiazole synthase (thiG) CSB-YP763289SSL
CSB-EP763289SSL
CSB-BP763289SSL
CSB-MP763289SSL
CSB-EP763289SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Protein grpE (grpE) CSB-YP763290SSL
CSB-EP763290SSL
CSB-BP763290SSL
CSB-MP763290SSL
CSB-EP763290SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP763291SZB
CSB-EP763291SZB
CSB-BP763291SZB
CSB-MP763291SZB
CSB-EP763291SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP763292SZB
CSB-EP763292SZB
CSB-BP763292SZB
CSB-MP763292SZB
CSB-EP763292SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfate/thiosulfate import ATP-binding protein CysA (cysA) CSB-YP763293SZB
CSB-EP763293SZB
CSB-BP763293SZB
CSB-MP763293SZB
CSB-EP763293SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Two-component-system connector protein SafA (safA), partial CSB-YP763294SZB
CSB-EP763294SZB
CSB-BP763294SZB
CSB-MP763294SZB
CSB-EP763294SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromosome partition protein mukE (mukE) CSB-YP763295SZB
CSB-EP763295SZB
CSB-BP763295SZB
CSB-MP763295SZB
CSB-EP763295SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA polymerase-associated protein RapA (rapA), partial CSB-YP763296SZB
CSB-EP763296SZB
CSB-BP763296SZB
CSB-MP763296SZB
CSB-EP763296SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP763297RDR
CSB-EP763297RDR
CSB-BP763297RDR
CSB-MP763297RDR
CSB-EP763297RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Ribonuclease H (rnhA) CSB-YP763298RDR
CSB-EP763298RDR
CSB-BP763298RDR
CSB-MP763298RDR
CSB-EP763298RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Alanine--tRNA ligase (alaS), partial CSB-YP763299RDR
CSB-EP763299RDR
CSB-BP763299RDR
CSB-MP763299RDR
CSB-EP763299RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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