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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Neurospora crassa Glutamyl-tRNA (Gln) amidotransferase subunit B, mitochondrial (NCU09025) CSB-YP763018NHA
CSB-EP763018NHA
CSB-BP763018NHA
CSB-MP763018NHA
CSB-EP763018NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Transcription elongation factor spt-5 (spt-5), partial CSB-YP763019NHA
CSB-EP763019NHA
CSB-BP763019NHA
CSB-MP763019NHA
CSB-EP763019NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Altered inheritance of mitochondria protein 31, mitochondrial (aim-31), partial CSB-YP763020NHA1
CSB-EP763020NHA1
CSB-BP763020NHA1
CSB-MP763020NHA1
CSB-EP763020NHA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Histone chaperone rtt-106 (rtt-106) CSB-YP763021NHA
CSB-EP763021NHA
CSB-BP763021NHA
CSB-MP763021NHA
CSB-EP763021NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa pH-response regulator protein palA/rim-20 (rim-20), partial CSB-YP763022NHA
CSB-EP763022NHA
CSB-BP763022NHA
CSB-MP763022NHA
CSB-EP763022NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Putative nicotinamide N-methyltransferase (nnt-1) CSB-YP763023NHA
CSB-EP763023NHA
CSB-BP763023NHA
CSB-MP763023NHA
CSB-EP763023NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Mitochondrial import inner membrane translocase subunit tim-16 (pam-16) CSB-YP763024NHA
CSB-EP763024NHA
CSB-BP763024NHA
CSB-MP763024NHA
CSB-EP763024NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Palmitoyltransferase PFA3 (pfa-3), partial CSB-YP763025NHA1
CSB-EP763025NHA1
CSB-BP763025NHA1
CSB-MP763025NHA1
CSB-EP763025NHA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Deoxyhypusine hydroxylase (lia-1) CSB-YP763026NHA
CSB-EP763026NHA
CSB-BP763026NHA
CSB-MP763026NHA
CSB-EP763026NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Mitochondria fission 1 protein (fis-1), partial CSB-YP763027NHA
CSB-EP763027NHA
CSB-BP763027NHA
CSB-MP763027NHA
CSB-EP763027NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Cytosolic Fe-S cluster assembly factor NBP35 (nbp-35) CSB-YP763028NHA
CSB-EP763028NHA
CSB-BP763028NHA
CSB-MP763028NHA
CSB-EP763028NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Pro-apoptotic serine protease nma-111 (nma-111), partial CSB-YP763029NHA
CSB-EP763029NHA
CSB-BP763029NHA
CSB-MP763029NHA
CSB-EP763029NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Actin cytoskeleton-regulatory complex protein end-3 (end-3) CSB-YP763030NHA
CSB-EP763030NHA
CSB-BP763030NHA
CSB-MP763030NHA
CSB-EP763030NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa ATP-dependent DNA helicase II subunit 1 (ku70), partial CSB-YP763031NHA
CSB-EP763031NHA
CSB-BP763031NHA
CSB-MP763031NHA
CSB-EP763031NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa GPI inositol-deacylase (bst-1), partial CSB-YP763032NHA
CSB-EP763032NHA
CSB-BP763032NHA
CSB-MP763032NHA
CSB-EP763032NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Pre-mRNA polyadenylation factor fip-1 (fip-1) CSB-YP763033NHA
CSB-EP763033NHA
CSB-BP763033NHA
CSB-MP763033NHA
CSB-EP763033NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa GDP-mannose transporter (vrg-4), partial CSB-YP763034NHA
CSB-EP763034NHA
CSB-BP763034NHA
CSB-MP763034NHA
CSB-EP763034NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Mitochondrial import inner membrane translocase subunit tim-13 (tim-13) CSB-YP763035NHA
CSB-EP763035NHA
CSB-BP763035NHA
CSB-MP763035NHA
CSB-EP763035NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Protein btn-1 (btn-1), partial CSB-YP763036NHA
CSB-EP763036NHA
CSB-BP763036NHA
CSB-MP763036NHA
CSB-EP763036NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Palmitoyltransferase PFA4 (pfa-4), partial CSB-YP763037NHA1
CSB-EP763037NHA1
CSB-BP763037NHA1
CSB-MP763037NHA1
CSB-EP763037NHA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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