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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Photorhabdus luminescens subsp. laumondii Lipid A export ATP-binding/permease protein MsbA (msbA), partial CSB-YP762818PIJ1
CSB-EP762818PIJ1
CSB-BP762818PIJ1
CSB-MP762818PIJ1
CSB-EP762818PIJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 23S rRNA (uracil (747)-C (5))-methyltransferase RlmC (rlmC) CSB-YP762819PIJ
CSB-EP762819PIJ
CSB-BP762819PIJ
CSB-MP762819PIJ
CSB-EP762819PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Deoxycytidine triphosphate deaminase (dcd) CSB-YP762820PIJ
CSB-EP762820PIJ
CSB-BP762820PIJ
CSB-MP762820PIJ
CSB-EP762820PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP762821PIJ
CSB-EP762821PIJ
CSB-BP762821PIJ
CSB-MP762821PIJ
CSB-EP762821PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Cell division protein ZipA homolog (zipA), partial CSB-YP762822PIJ
CSB-EP762822PIJ
CSB-BP762822PIJ
CSB-MP762822PIJ
CSB-EP762822PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Glutamine--tRNA ligase (glnS), partial CSB-YP762823PIJ
CSB-EP762823PIJ
CSB-BP762823PIJ
CSB-MP762823PIJ
CSB-EP762823PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Octanoyltransferase (lipB) CSB-YP762824PIJ
CSB-EP762824PIJ
CSB-BP762824PIJ
CSB-MP762824PIJ
CSB-EP762824PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Glutamate--cysteine ligase (gshA), partial CSB-YP762825PIJ
CSB-EP762825PIJ
CSB-BP762825PIJ
CSB-MP762825PIJ
CSB-EP762825PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii UPF0255 protein plu1242 (plu1242) CSB-YP762826PIJ
CSB-EP762826PIJ
CSB-BP762826PIJ
CSB-MP762826PIJ
CSB-EP762826PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Putative Holliday junction resolvase (plu1182) CSB-YP762827PIJ
CSB-EP762827PIJ
CSB-BP762827PIJ
CSB-MP762827PIJ
CSB-EP762827PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii D-erythrose-4-phosphate dehydrogenase (epd) CSB-YP762828PIJ
CSB-EP762828PIJ
CSB-BP762828PIJ
CSB-MP762828PIJ
CSB-EP762828PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP762829PIJ
CSB-EP762829PIJ
CSB-BP762829PIJ
CSB-MP762829PIJ
CSB-EP762829PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP762830PIJ
CSB-EP762830PIJ
CSB-BP762830PIJ
CSB-MP762830PIJ
CSB-EP762830PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Siroheme synthase (cysG) CSB-YP762831PIJ
CSB-EP762831PIJ
CSB-BP762831PIJ
CSB-MP762831PIJ
CSB-EP762831PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP762832PIJ
CSB-EP762832PIJ
CSB-BP762832PIJ
CSB-MP762832PIJ
CSB-EP762832PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Outer membrane protein assembly factor YaeT (yaeT), partial CSB-YP762833PIJ
CSB-EP762833PIJ
CSB-BP762833PIJ
CSB-MP762833PIJ
CSB-EP762833PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP762834PIJ
CSB-EP762834PIJ
CSB-BP762834PIJ
CSB-MP762834PIJ
CSB-EP762834PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii RNA polymerase-associated protein RapA (rapA), partial CSB-YP762835PIJ
CSB-EP762835PIJ
CSB-BP762835PIJ
CSB-MP762835PIJ
CSB-EP762835PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Carbamoyl-phosphate synthase small chain (carA) CSB-YP762836PIJ
CSB-EP762836PIJ
CSB-BP762836PIJ
CSB-MP762836PIJ
CSB-EP762836PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 30S ribosomal protein S20 (rpsT) CSB-YP762837PIJ
CSB-EP762837PIJ
CSB-BP762837PIJ
CSB-MP762837PIJ
CSB-EP762837PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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