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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio vulnificus 3-isopropylmalate dehydrogenase (leuB) CSB-YP762718VCQ
CSB-EP762718VCQ
CSB-BP762718VCQ
CSB-MP762718VCQ
CSB-EP762718VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus GTPase obg (obg) CSB-YP762719VCQ
CSB-EP762719VCQ
CSB-BP762719VCQ
CSB-MP762719VCQ
CSB-EP762719VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP762720VCQ
CSB-EP762720VCQ
CSB-BP762720VCQ
CSB-MP762720VCQ
CSB-EP762720VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 50S ribosomal protein L17 (rplQ) CSB-YP762721VCQ
CSB-EP762721VCQ
CSB-BP762721VCQ
CSB-MP762721VCQ
CSB-EP762721VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 50S ribosomal protein L18 (rplR) CSB-YP762722VCQ
CSB-EP762722VCQ
CSB-BP762722VCQ
CSB-MP762722VCQ
CSB-EP762722VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 50S ribosomal protein L29 (rpmC) CSB-YP762723VCQ
CSB-EP762723VCQ
CSB-BP762723VCQ
CSB-MP762723VCQ
CSB-EP762723VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 50S ribosomal protein L4 (rplD) CSB-YP762724VCQ
CSB-EP762724VCQ
CSB-BP762724VCQ
CSB-MP762724VCQ
CSB-EP762724VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 3-deoxy-D-manno-octulosonic acid kinase (kdkA) CSB-YP762725VCQ
CSB-EP762725VCQ
CSB-BP762725VCQ
CSB-MP762725VCQ
CSB-EP762725VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Ribonuclease PH (rph) CSB-YP762726VCQ
CSB-EP762726VCQ
CSB-BP762726VCQ
CSB-MP762726VCQ
CSB-EP762726VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus D-tyrosyl-tRNA (Tyr) deacylase (dtd) CSB-YP762727VCQ
CSB-EP762727VCQ
CSB-BP762727VCQ
CSB-MP762727VCQ
CSB-EP762727VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Sec-independent protein translocase protein TatA (tatA), partial CSB-YP762728VCQ
CSB-EP762728VCQ
CSB-BP762728VCQ
CSB-MP762728VCQ
CSB-EP762728VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus LexA repressor (lexA) CSB-YP762729VCQ
CSB-EP762729VCQ
CSB-BP762729VCQ
CSB-MP762729VCQ
CSB-EP762729VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Porphobilinogen deaminase (hemC) CSB-YP762730VCQ
CSB-EP762730VCQ
CSB-BP762730VCQ
CSB-MP762730VCQ
CSB-EP762730VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP762731VCQ
CSB-EP762731VCQ
CSB-BP762731VCQ
CSB-MP762731VCQ
CSB-EP762731VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-ribosylhomocysteine lyase (luxS) CSB-YP762732WBQ
CSB-EP762732WBQ
CSB-BP762732WBQ
CSB-MP762732WBQ
CSB-EP762732WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP762733WBQ
CSB-EP762733WBQ
CSB-BP762733WBQ
CSB-MP762733WBQ
CSB-EP762733WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Outer-membrane lipoprotein carrier protein (lolA) CSB-YP762734WBQ
CSB-EP762734WBQ
CSB-BP762734WBQ
CSB-MP762734WBQ
CSB-EP762734WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type III pantothenate kinase (coaX) CSB-YP762735WBQ
CSB-EP762735WBQ
CSB-BP762735WBQ
CSB-MP762735WBQ
CSB-EP762735WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable rRNA maturation factor (WS0555) CSB-YP762736WBQ
CSB-EP762736WBQ
CSB-BP762736WBQ
CSB-MP762736WBQ
CSB-EP762736WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP762737WBQ
CSB-EP762737WBQ
CSB-BP762737WBQ
CSB-MP762737WBQ
CSB-EP762737WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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