Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ribonuclease Y (rny), partial CSB-YP762638WBQ
CSB-EP762638WBQ
CSB-BP762638WBQ
CSB-MP762638WBQ
CSB-EP762638WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis tRNA dimethylallyltransferase 2 (miaA2) CSB-YP762639EYA
CSB-EP762639EYA
CSB-BP762639EYA
CSB-MP762639EYA
CSB-EP762639EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii tRNA dimethylallyltransferase (miaA) CSB-YP762640PIJ
CSB-EP762640PIJ
CSB-BP762640PIJ
CSB-MP762640PIJ
CSB-EP762640PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii tRNA pseudouridine synthase A (truA) CSB-YP762641PIJ
CSB-EP762641PIJ
CSB-BP762641PIJ
CSB-MP762641PIJ
CSB-EP762641PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP762642PIJ
CSB-EP762642PIJ
CSB-BP762642PIJ
CSB-MP762642PIJ
CSB-EP762642PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus 60 kDa chaperonin 1 (groL1), partial CSB-YP762643GCI
CSB-EP762643GCI
CSB-BP762643GCI
CSB-MP762643GCI
CSB-EP762643GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Multifunctional CCA protein (cca) CSB-YP762644CKA
CSB-EP762644CKA
CSB-BP762644CKA
CSB-MP762644CKA
CSB-EP762644CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus PTS system N-acetylmuramic acid-specific EIIBC component (murP), partial CSB-YP762645VCQ
CSB-EP762645VCQ
CSB-BP762645VCQ
CSB-MP762645VCQ
CSB-EP762645VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Peptide deformylase 1 (def1) CSB-YP762646VCQ
CSB-EP762646VCQ
CSB-BP762646VCQ
CSB-MP762646VCQ
CSB-EP762646VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus N-acetylneuraminate epimerase (nanM) CSB-YP762647VCQ
CSB-EP762647VCQ
CSB-BP762647VCQ
CSB-MP762647VCQ
CSB-EP762647VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Peptidase T (pepT) CSB-YP762648VCQ
CSB-EP762648VCQ
CSB-BP762648VCQ
CSB-MP762648VCQ
CSB-EP762648VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Putative reductase VVA0771 (VVA0771) CSB-YP762649VCQ
CSB-EP762649VCQ
CSB-BP762649VCQ
CSB-MP762649VCQ
CSB-EP762649VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP762650VCQ
CSB-EP762650VCQ
CSB-BP762650VCQ
CSB-MP762650VCQ
CSB-EP762650VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Betaine aldehyde dehydrogenase (betB) CSB-YP762651VCQ
CSB-EP762651VCQ
CSB-BP762651VCQ
CSB-MP762651VCQ
CSB-EP762651VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Maltose transport system permease protein malG (malG), partial CSB-YP762652VCQ1
CSB-EP762652VCQ1
CSB-BP762652VCQ1
CSB-MP762652VCQ1
CSB-EP762652VCQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Catalase (VVA0294) CSB-YP762653VCQ
CSB-EP762653VCQ
CSB-BP762653VCQ
CSB-MP762653VCQ
CSB-EP762653VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Beta-galactosidase (lacZ), partial CSB-YP762654VCQ
CSB-EP762654VCQ
CSB-BP762654VCQ
CSB-MP762654VCQ
CSB-EP762654VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Pyridoxamine kinase (pdxY) CSB-YP762655VCQ
CSB-EP762655VCQ
CSB-BP762655VCQ
CSB-MP762655VCQ
CSB-EP762655VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus ATP synthase subunit a (atpB), partial CSB-YP762656VCQ1
CSB-EP762656VCQ1
CSB-BP762656VCQ1
CSB-MP762656VCQ1
CSB-EP762656VCQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Ribosomal RNA small subunit methyltransferase B (rsmB) CSB-YP762657VCQ
CSB-EP762657VCQ
CSB-BP762657VCQ
CSB-MP762657VCQ
CSB-EP762657VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>