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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Trehalose-phosphate phosphatase (otsB) CSB-YP763220MVH
CSB-EP763220MVH
CSB-BP763220MVH
CSB-MP763220MVH
CSB-EP763220MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant F420-0:gamma-glutamyl ligase (cofE) CSB-YP763221MVH
CSB-EP763221MVH
CSB-BP763221MVH
CSB-MP763221MVH
CSB-EP763221MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Molybdenum cofactor biosynthesis protein A 1 (moaA1) CSB-YP763222MVH
CSB-EP763222MVH
CSB-BP763222MVH
CSB-MP763222MVH
CSB-EP763222MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Inactive PGL/p-HBAD biosynthesis glycosyltransferase Mb2982c (Mb2982c) CSB-YP763223MVH
CSB-EP763223MVH
CSB-BP763223MVH
CSB-MP763223MVH
CSB-EP763223MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsD (ppsD), partial CSB-YP763224MVH
CSB-EP763224MVH
CSB-BP763224MVH
CSB-MP763224MVH
CSB-EP763224MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydrodipicolinate reductase (dapB) CSB-YP763225MVH
CSB-EP763225MVH
CSB-BP763225MVH
CSB-MP763225MVH
CSB-EP763225MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate--tRNA ligase (aspS), partial CSB-YP763226MVH
CSB-EP763226MVH
CSB-BP763226MVH
CSB-MP763226MVH
CSB-EP763226MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfite reductase [ferredoxin] (sir), partial CSB-YP763227MVH
CSB-EP763227MVH
CSB-BP763227MVH
CSB-MP763227MVH
CSB-EP763227MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized transporter Mb2001c (Mb2001c), partial CSB-YP763228MVH
CSB-EP763228MVH
CSB-BP763228MVH
CSB-MP763228MVH
CSB-EP763228MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable enoyl-CoA hydratase echA12 (echA12) CSB-YP763229MVH
CSB-EP763229MVH
CSB-BP763229MVH
CSB-MP763229MVH
CSB-EP763229MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alpha-pyrone synthesis polyketide synthase-like Pks18 (pks18) CSB-YP763230MVH
CSB-EP763230MVH
CSB-BP763230MVH
CSB-MP763230MVH
CSB-EP763230MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP763231MVH
CSB-EP763231MVH
CSB-BP763231MVH
CSB-MP763231MVH
CSB-EP763231MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative lipoprotein lpqT (lpqT) CSB-YP763232MVH
CSB-EP763232MVH
CSB-BP763232MVH
CSB-MP763232MVH
CSB-EP763232MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Succinyl-CoA ligase [ADP-forming] subunit alpha (sucD) CSB-YP763233MVH
CSB-EP763233MVH
CSB-BP763233MVH
CSB-MP763233MVH
CSB-EP763233MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exopolysaccharide phosphotransferase CpsY (cpsY), partial CSB-YP763234MVH
CSB-EP763234MVH
CSB-BP763234MVH
CSB-MP763234MVH
CSB-EP763234MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0336 protein Mb0656 (Mb0656) CSB-YP763235MVH
CSB-EP763235MVH
CSB-BP763235MVH
CSB-MP763235MVH
CSB-EP763235MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide deformylase (def) CSB-YP763236MVH
CSB-EP763236MVH
CSB-BP763236MVH
CSB-MP763236MVH
CSB-EP763236MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative S-adenosyl-L-methionine-dependent methyltransferase Mb0151 (Mb0151) CSB-YP763237MVH
CSB-EP763237MVH
CSB-BP763237MVH
CSB-MP763237MVH
CSB-EP763237MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA replication and repair protein recF (recF) CSB-YP763238MVH
CSB-EP763238MVH
CSB-BP763238MVH
CSB-MP763238MVH
CSB-EP763238MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus tRNA dimethylallyltransferase (miaA) CSB-YP763239HCO
CSB-EP763239HCO
CSB-BP763239HCO
CSB-MP763239HCO
CSB-EP763239HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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