重組蛋白
重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實(shí)驗(yàn)室中使用了大量的重組蛋白質(zhì),包括商業(yè)蛋白質(zhì)和在特定研究項(xiàng)目中生成的蛋白質(zhì)。它們?cè)谠S多應(yīng)用中發(fā)揮著關(guān)鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量(驗(yàn)證的生物活性,較低的內(nèi)毒素水平和高純度)的重組蛋白質(zhì)來(lái)支持您的研究,您來(lái)對(duì)地方了。
經(jīng)過(guò)16多年的發(fā)展,華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng),用于表達(dá)重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達(dá)和純化技術(shù),包括基于桿狀病毒外膜蛋白(OMP)的蛋白質(zhì)表達(dá)技術(shù),以及膜蛋白表達(dá)技術(shù)等。
產(chǎn)品特點(diǎn)
- 超過(guò)10,000種重組蛋白覆蓋大多數(shù)基因/靶點(diǎn);
- 5種表達(dá)系統(tǒng),包括大腸桿菌(E.coli)、酵母、桿狀病毒感染的昆蟲細(xì)胞、哺乳動(dòng)物細(xì)胞和體外大腸桿菌。而體外大腸桿菌表達(dá)系統(tǒng)是華美生物的獨(dú)特表達(dá)系統(tǒng)。與傳統(tǒng)蛋白表達(dá)系統(tǒng)相比,體外大腸桿菌省略了許多過(guò)程,如質(zhì)粒轉(zhuǎn)化、細(xì)胞培養(yǎng)、收集、破碎和離心等,大大提高了工作效率。
- 多種標(biāo)簽,包括His、GST、Flag、MBP等。
- 廣泛應(yīng)用,包括抗原、細(xì)胞分析、結(jié)合分析/蛋白質(zhì)相互作用、細(xì)胞培養(yǎng)-無(wú)血清培養(yǎng)基、藥物相關(guān)研究、體外酶活性、ELISA標(biāo)準(zhǔn)及其原料、體內(nèi)研究、質(zhì)譜標(biāo)準(zhǔn)、蛋白芯片、SDS-PAGE控制、蛋白質(zhì)結(jié)構(gòu)分析(晶體/電子顯微鏡)。
- 多種物種,包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無(wú)動(dòng)物副產(chǎn)品的動(dòng)物源性重組蛋白質(zhì)。華美生物已開發(fā)出無(wú)動(dòng)物副產(chǎn)品接觸的重組蛋白質(zhì)產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì),或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體,請(qǐng)查看華美生物蛋白質(zhì)定制服務(wù)。
通過(guò)選擇目標(biāo)名稱的首字母瀏覽產(chǎn)品。
產(chǎn)品名稱 | 貨號(hào) | 規(guī)格 |
---|---|---|
Recombinant Photobacterium profundum 30S ribosomal protein S12 (rpsL) | CSB-YP764421PIG CSB-EP764421PIG CSB-BP764421PIG CSB-MP764421PIG CSB-EP764421PIG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Photobacterium profundum Argininosuccinate synthase (argG) | CSB-YP764422PIG CSB-EP764422PIG CSB-BP764422PIG CSB-MP764422PIG CSB-EP764422PIG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Photobacterium profundum ATP-dependent protease ATPase subunit HslU (hslU) | CSB-YP764423PIG CSB-EP764423PIG CSB-BP764423PIG CSB-MP764423PIG CSB-EP764423PIG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Photobacterium profundum 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial | CSB-YP764424PIG CSB-EP764424PIG CSB-BP764424PIG CSB-MP764424PIG CSB-EP764424PIG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Photobacterium profundum Protein CrcB homolog (crcB), partial | CSB-YP764425PIG1 CSB-EP764425PIG1 CSB-BP764425PIG1 CSB-MP764425PIG1 CSB-EP764425PIG1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Methanococcus maripaludis UPF0290 protein MMP1698 (MMP1698), partial | CSB-YP764426MSB1 CSB-EP764426MSB1 CSB-BP764426MSB1 CSB-MP764426MSB1 CSB-EP764426MSB1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Methanococcus maripaludis Digeranylgeranylglyceryl phosphate synthase (MMP1677), partial | CSB-YP764427MSB CSB-EP764427MSB CSB-BP764427MSB CSB-MP764427MSB CSB-EP764427MSB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Methanococcus maripaludis Tetrahydromethanopterin S-methyltransferase subunit A (mtrA), partial | CSB-YP764428MSB CSB-EP764428MSB CSB-BP764428MSB CSB-MP764428MSB CSB-EP764428MSB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Methanococcus maripaludis 50S ribosomal protein L3P (rpl3p) | CSB-YP764429MSB CSB-EP764429MSB CSB-BP764429MSB CSB-MP764429MSB CSB-EP764429MSB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Methanococcus maripaludis 5-methylthioadenosine/S-adenosylhomocysteine deaminase (mtaD) | CSB-YP764430MSB CSB-EP764430MSB CSB-BP764430MSB CSB-MP764430MSB CSB-EP764430MSB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Methanococcus maripaludis UPF0597 protein MMP1468 (MMP1468) | CSB-YP764431MSB CSB-EP764431MSB CSB-BP764431MSB CSB-MP764431MSB CSB-EP764431MSB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Methanococcus maripaludis tRNA (cytidine (56)-2'-O)-methyltransferase (MMP1425) | CSB-YP764432MSB CSB-EP764432MSB CSB-BP764432MSB CSB-MP764432MSB CSB-EP764432MSB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Methanococcus maripaludis 50S ribosomal protein L6P (rpl6p) | CSB-YP764433MSB CSB-EP764433MSB CSB-BP764433MSB CSB-MP764433MSB CSB-EP764433MSB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Methanococcus maripaludis 50S ribosomal protein L14P (rpl14p) | CSB-YP764434MSB CSB-EP764434MSB CSB-BP764434MSB CSB-MP764434MSB CSB-EP764434MSB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Methanococcus maripaludis tRNA-splicing ligase RtcB (rtcB) | CSB-YP764435MSB CSB-EP764435MSB CSB-BP764435MSB CSB-MP764435MSB CSB-EP764435MSB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Methanococcus maripaludis 30S ribosomal protein S7P (rps7p) | CSB-YP764436MSB CSB-EP764436MSB CSB-BP764436MSB CSB-MP764436MSB CSB-EP764436MSB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Methanococcus maripaludis IMP cyclohydrolase (purO) | CSB-YP764437MSB CSB-EP764437MSB CSB-BP764437MSB CSB-MP764437MSB CSB-EP764437MSB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Methanococcus maripaludis UPF0333 protein MMP1283 (MMP1283), partial | CSB-YP764438MSB1 CSB-EP764438MSB1 CSB-BP764438MSB1 CSB-MP764438MSB1 CSB-EP764438MSB1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Methanococcus maripaludis UPF0280 protein MMP1236 (MMP1236) | CSB-YP764439MSB CSB-EP764439MSB CSB-BP764439MSB CSB-MP764439MSB CSB-EP764439MSB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Methanococcus maripaludis Hydroxyethylthiazole kinase (thiM) | CSB-YP764440MSB CSB-EP764440MSB CSB-BP764440MSB CSB-MP764440MSB CSB-EP764440MSB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。
第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條:如何選擇合適的表達(dá)載體?
表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。
根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022