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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Photobacterium profundum 30S ribosomal protein S12 (rpsL) CSB-YP764421PIG
CSB-EP764421PIG
CSB-BP764421PIG
CSB-MP764421PIG
CSB-EP764421PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Argininosuccinate synthase (argG) CSB-YP764422PIG
CSB-EP764422PIG
CSB-BP764422PIG
CSB-MP764422PIG
CSB-EP764422PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP764423PIG
CSB-EP764423PIG
CSB-BP764423PIG
CSB-MP764423PIG
CSB-EP764423PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP764424PIG
CSB-EP764424PIG
CSB-BP764424PIG
CSB-MP764424PIG
CSB-EP764424PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Protein CrcB homolog (crcB), partial CSB-YP764425PIG1
CSB-EP764425PIG1
CSB-BP764425PIG1
CSB-MP764425PIG1
CSB-EP764425PIG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0290 protein MMP1698 (MMP1698), partial CSB-YP764426MSB1
CSB-EP764426MSB1
CSB-BP764426MSB1
CSB-MP764426MSB1
CSB-EP764426MSB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Digeranylgeranylglyceryl phosphate synthase (MMP1677), partial CSB-YP764427MSB
CSB-EP764427MSB
CSB-BP764427MSB
CSB-MP764427MSB
CSB-EP764427MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Tetrahydromethanopterin S-methyltransferase subunit A (mtrA), partial CSB-YP764428MSB
CSB-EP764428MSB
CSB-BP764428MSB
CSB-MP764428MSB
CSB-EP764428MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L3P (rpl3p) CSB-YP764429MSB
CSB-EP764429MSB
CSB-BP764429MSB
CSB-MP764429MSB
CSB-EP764429MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 5-methylthioadenosine/S-adenosylhomocysteine deaminase (mtaD) CSB-YP764430MSB
CSB-EP764430MSB
CSB-BP764430MSB
CSB-MP764430MSB
CSB-EP764430MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0597 protein MMP1468 (MMP1468) CSB-YP764431MSB
CSB-EP764431MSB
CSB-BP764431MSB
CSB-MP764431MSB
CSB-EP764431MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis tRNA (cytidine (56)-2'-O)-methyltransferase (MMP1425) CSB-YP764432MSB
CSB-EP764432MSB
CSB-BP764432MSB
CSB-MP764432MSB
CSB-EP764432MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L6P (rpl6p) CSB-YP764433MSB
CSB-EP764433MSB
CSB-BP764433MSB
CSB-MP764433MSB
CSB-EP764433MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L14P (rpl14p) CSB-YP764434MSB
CSB-EP764434MSB
CSB-BP764434MSB
CSB-MP764434MSB
CSB-EP764434MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis tRNA-splicing ligase RtcB (rtcB) CSB-YP764435MSB
CSB-EP764435MSB
CSB-BP764435MSB
CSB-MP764435MSB
CSB-EP764435MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S7P (rps7p) CSB-YP764436MSB
CSB-EP764436MSB
CSB-BP764436MSB
CSB-MP764436MSB
CSB-EP764436MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis IMP cyclohydrolase (purO) CSB-YP764437MSB
CSB-EP764437MSB
CSB-BP764437MSB
CSB-MP764437MSB
CSB-EP764437MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0333 protein MMP1283 (MMP1283), partial CSB-YP764438MSB1
CSB-EP764438MSB1
CSB-BP764438MSB1
CSB-MP764438MSB1
CSB-EP764438MSB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0280 protein MMP1236 (MMP1236) CSB-YP764439MSB
CSB-EP764439MSB
CSB-BP764439MSB
CSB-MP764439MSB
CSB-EP764439MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Hydroxyethylthiazole kinase (thiM) CSB-YP764440MSB
CSB-EP764440MSB
CSB-BP764440MSB
CSB-MP764440MSB
CSB-EP764440MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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