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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 6-carboxyhexanoate--CoA ligase (bioW) CSB-YP764621CQF
CSB-EP764621CQF
CSB-BP764621CQF
CSB-MP764621CQF
CSB-EP764621CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative Holliday junction resolvase (DIP1349) CSB-YP764622CQF
CSB-EP764622CQF
CSB-BP764622CQF
CSB-MP764622CQF
CSB-EP764622CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate carbamoyltransferase (pyrB) CSB-YP764623CQF
CSB-EP764623CQF
CSB-BP764623CQF
CSB-MP764623CQF
CSB-EP764623CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0042 nucleotide-binding protein DIP1313 (DIP1313) CSB-YP764624CQF
CSB-EP764624CQF
CSB-BP764624CQF
CSB-MP764624CQF
CSB-EP764624CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant AAA ATPase forming ring-shaped complexes (arc), partial CSB-YP764625CQF
CSB-EP764625CQF
CSB-BP764625CQF
CSB-MP764625CQF
CSB-EP764625CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L35 (rpmI) CSB-YP764626CQF
CSB-EP764626CQF
CSB-BP764626CQF
CSB-MP764626CQF
CSB-EP764626CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Biotin synthase 2 (bioB2) CSB-YP764627CQF
CSB-EP764627CQF
CSB-BP764627CQF
CSB-MP764627CQF
CSB-EP764627CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) CSB-YP764628CQF
CSB-EP764628CQF
CSB-BP764628CQF
CSB-MP764628CQF
CSB-EP764628CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit beta (atpD) CSB-YP764629CQF
CSB-EP764629CQF
CSB-BP764629CQF
CSB-MP764629CQF
CSB-EP764629CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional repressor of iron proteins A (ripA) CSB-YP764630CQF
CSB-EP764630CQF
CSB-BP764630CQF
CSB-MP764630CQF
CSB-EP764630CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP764631CQF
CSB-EP764631CQF
CSB-BP764631CQF
CSB-MP764631CQF
CSB-EP764631CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP764632CQF
CSB-EP764632CQF
CSB-BP764632CQF
CSB-MP764632CQF
CSB-EP764632CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide chain release factor 2 (prfB) CSB-YP764633CQF
CSB-EP764633CQF
CSB-BP764633CQF
CSB-MP764633CQF
CSB-EP764633CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homoserine O-acetyltransferase (metX) CSB-YP764634CQF
CSB-EP764634CQF
CSB-BP764634CQF
CSB-MP764634CQF
CSB-EP764634CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L13 (rplM) CSB-YP764635CQF
CSB-EP764635CQF
CSB-BP764635CQF
CSB-MP764635CQF
CSB-EP764635CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S13 (rpsM) CSB-YP764636CQF
CSB-EP764636CQF
CSB-BP764636CQF
CSB-MP764636CQF
CSB-EP764636CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor Tu (tuf) CSB-YP764637CQF
CSB-EP764637CQF
CSB-BP764637CQF
CSB-MP764637CQF
CSB-EP764637CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L10 (rplJ) CSB-YP764638CQF
CSB-EP764638CQF
CSB-BP764638CQF
CSB-MP764638CQF
CSB-EP764638CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP764639CQF
CSB-EP764639CQF
CSB-BP764639CQF
CSB-MP764639CQF
CSB-EP764639CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA replication and repair protein recF (recF) CSB-YP764640CQF
CSB-EP764640CQF
CSB-BP764640CQF
CSB-MP764640CQF
CSB-EP764640CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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