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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Oryza sativa subsp. japonica Putative germin-like protein 2-2 (Os02g0491700, LOC_Os02g29010) CSB-YP764281OFG
CSB-EP764281OFG
CSB-BP764281OFG
CSB-MP764281OFG
CSB-EP764281OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Leucine aminopeptidase 2, chloroplastic (Os02g0794700, LOC_Os02g55140), partial CSB-YP764282OFG
CSB-EP764282OFG
CSB-BP764282OFG
CSB-MP764282OFG
CSB-EP764282OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable protein phosphatase 2C 14 (Os02g0471500, LOC_Os02g27220), partial CSB-YP764283OFG
CSB-EP764283OFG
CSB-BP764283OFG
CSB-MP764283OFG
CSB-EP764283OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica 18.6 kDa class III heat shock protein (HSP18.6) CSB-YP764284OFG
CSB-EP764284OFG
CSB-BP764284OFG
CSB-MP764284OFG
CSB-EP764284OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Zinc finger CCCH domain-containing protein 19 (Os02g0831100, LOC_Os02g58440), partial CSB-YP764285OFG
CSB-EP764285OFG
CSB-BP764285OFG
CSB-MP764285OFG
CSB-EP764285OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Multivesicular body subunit 12B (Fam125b) CSB-YP764286MO
CSB-EP764286MO
CSB-BP764286MO
CSB-MP764286MO
CSB-EP764286MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans FK506-binding protein 1A (fprA) CSB-YP764287EKF
CSB-EP764287EKF
CSB-BP764287EKF
CSB-MP764287EKF
CSB-EP764287EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein RecA (recA) CSB-YP764288EEG
CSB-EP764288EEG
CSB-BP764288EEG
CSB-MP764288EEG
CSB-EP764288EEG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homarus americanus Pseudohemocyanin-1 (phc-1), partial CSB-YP764289HWO
CSB-EP764289HWO
CSB-BP764289HWO
CSB-MP764289HWO
CSB-EP764289HWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP764290MAAC
CSB-EP764290MAAC
CSB-BP764290MAAC
CSB-MP764290MAAC
CSB-EP764290MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile 50S ribosomal protein L33 (rpmG) CSB-YP764291MAAC
CSB-EP764291MAAC
CSB-BP764291MAAC
CSB-MP764291MAAC
CSB-EP764291MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile Segregation and condensation protein A (scpA) CSB-YP764292MAAC
CSB-EP764292MAAC
CSB-BP764292MAAC
CSB-MP764292MAAC
CSB-EP764292MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile Alanine--tRNA ligase (alaS), partial CSB-YP764293MAAC
CSB-EP764293MAAC
CSB-BP764293MAAC
CSB-MP764293MAAC
CSB-EP764293MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP764294MAAC
CSB-EP764294MAAC
CSB-BP764294MAAC
CSB-MP764294MAAC
CSB-EP764294MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP764295MAAC
CSB-EP764295MAAC
CSB-BP764295MAAC
CSB-MP764295MAAC
CSB-EP764295MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile 50S ribosomal protein L32 (rpmF) CSB-YP764296MAAC
CSB-EP764296MAAC
CSB-BP764296MAAC
CSB-MP764296MAAC
CSB-EP764296MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile 50S ribosomal protein L27 (rpmA) CSB-YP764297MAAC
CSB-EP764297MAAC
CSB-BP764297MAAC
CSB-MP764297MAAC
CSB-EP764297MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile 50S ribosomal protein L5 (rplE) CSB-YP764298MAAC
CSB-EP764298MAAC
CSB-BP764298MAAC
CSB-MP764298MAAC
CSB-EP764298MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile 30S ribosomal protein S10 (rpsJ) CSB-YP764299MAAC
CSB-EP764299MAAC
CSB-BP764299MAAC
CSB-MP764299MAAC
CSB-EP764299MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile ATP synthase subunit c (atpE), partial CSB-YP764300MAAC1
CSB-EP764300MAAC1
CSB-BP764300MAAC1
CSB-MP764300MAAC1
CSB-EP764300MAAC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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