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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Protochlamydia amoebophila UvrABC system protein C (uvrC), partial CSB-YP764481PAAD
CSB-EP764481PAAD
CSB-BP764481PAAD
CSB-MP764481PAAD
CSB-EP764481PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila LL-diaminopimelate aminotransferase (dapL) CSB-YP764482PAAD
CSB-EP764482PAAD
CSB-BP764482PAAD
CSB-MP764482PAAD
CSB-EP764482PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP764483PAAD
CSB-EP764483PAAD
CSB-BP764483PAAD
CSB-MP764483PAAD
CSB-EP764483PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 50S ribosomal protein L31 type B (rpmE2) CSB-YP764484PAAD
CSB-EP764484PAAD
CSB-BP764484PAAD
CSB-MP764484PAAD
CSB-EP764484PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP764485PAAD
CSB-EP764485PAAD
CSB-BP764485PAAD
CSB-MP764485PAAD
CSB-EP764485PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP764486PAAD
CSB-EP764486PAAD
CSB-BP764486PAAD
CSB-MP764486PAAD
CSB-EP764486PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 30S ribosomal protein S21 (rpsU) CSB-YP764487PAAD
CSB-EP764487PAAD
CSB-BP764487PAAD
CSB-MP764487PAAD
CSB-EP764487PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 50S ribosomal protein L6 (rplF) CSB-YP764488PAAD
CSB-EP764488PAAD
CSB-BP764488PAAD
CSB-MP764488PAAD
CSB-EP764488PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 50S ribosomal protein L24 (rplX) CSB-YP764489PAAD
CSB-EP764489PAAD
CSB-BP764489PAAD
CSB-MP764489PAAD
CSB-EP764489PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 50S ribosomal protein L3 (rplC) CSB-YP764490PAAD
CSB-EP764490PAAD
CSB-BP764490PAAD
CSB-MP764490PAAD
CSB-EP764490PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Aspartate--tRNA ligase (aspS), partial CSB-YP764491PAAD
CSB-EP764491PAAD
CSB-BP764491PAAD
CSB-MP764491PAAD
CSB-EP764491PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Ribosomal RNA large subunit methyltransferase N 1 (rlmN1) CSB-YP764492PAAD
CSB-EP764492PAAD
CSB-BP764492PAAD
CSB-MP764492PAAD
CSB-EP764492PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP764493PAAD
CSB-EP764493PAAD
CSB-BP764493PAAD
CSB-MP764493PAAD
CSB-EP764493PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP764494PAAD
CSB-EP764494PAAD
CSB-BP764494PAAD
CSB-MP764494PAAD
CSB-EP764494PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Elongation factor G (fusA), partial CSB-YP764495PAAD
CSB-EP764495PAAD
CSB-BP764495PAAD
CSB-MP764495PAAD
CSB-EP764495PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP764496PAAD
CSB-EP764496PAAD
CSB-BP764496PAAD
CSB-MP764496PAAD
CSB-EP764496PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 30S ribosomal protein S20 (rpsT) CSB-YP764497PAAD
CSB-EP764497PAAD
CSB-BP764497PAAD
CSB-MP764497PAAD
CSB-EP764497PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP764498PAAD
CSB-EP764498PAAD
CSB-BP764498PAAD
CSB-MP764498PAAD
CSB-EP764498PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Guanine nucleotide-binding protein-like 1 (Gnl1) CSB-YP764499RA
CSB-EP764499RA
CSB-BP764499RA
CSB-MP764499RA
CSB-EP764499RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Uncharacterized protein C6orf136 homolog CSB-YP764500RA
CSB-EP764500RA
CSB-BP764500RA
CSB-MP764500RA
CSB-EP764500RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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