Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Photobacterium profundum Recombination protein RecR (recR) CSB-YP764401PIG
CSB-EP764401PIG
CSB-BP764401PIG
CSB-MP764401PIG
CSB-EP764401PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Flagellar L-ring protein (flgH) CSB-YP764402PIG
CSB-EP764402PIG
CSB-BP764402PIG
CSB-MP764402PIG
CSB-EP764402PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP764403PIG
CSB-EP764403PIG
CSB-BP764403PIG
CSB-MP764403PIG
CSB-EP764403PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Na (+)-translocating NADH-quinone reductase subunit E (nqrE), partial CSB-YP764404PIG1
CSB-EP764404PIG1
CSB-BP764404PIG1
CSB-MP764404PIG1
CSB-EP764404PIG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Membrane-bound lytic murein transglycosylase F (mltF) CSB-YP764405PIG
CSB-EP764405PIG
CSB-BP764405PIG
CSB-MP764405PIG
CSB-EP764405PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP764406PIG
CSB-EP764406PIG
CSB-BP764406PIG
CSB-MP764406PIG
CSB-EP764406PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Protein grpE (grpE) CSB-YP764407PIG
CSB-EP764407PIG
CSB-BP764407PIG
CSB-MP764407PIG
CSB-EP764407PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 30S ribosomal protein S20 (rpsT) CSB-YP764408PIG
CSB-EP764408PIG
CSB-BP764408PIG
CSB-MP764408PIG
CSB-EP764408PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum UPF0246 protein PBPRA0561 (PBPRA0561) CSB-YP764409PIG
CSB-EP764409PIG
CSB-BP764409PIG
CSB-MP764409PIG
CSB-EP764409PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Vitamin B12-binding protein (btuF) CSB-YP764410PIG
CSB-EP764410PIG
CSB-BP764410PIG
CSB-MP764410PIG
CSB-EP764410PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP764411PIG
CSB-EP764411PIG
CSB-BP764411PIG
CSB-MP764411PIG
CSB-EP764411PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Multifunctional CCA protein (cca) CSB-YP764412PIG
CSB-EP764412PIG
CSB-BP764412PIG
CSB-MP764412PIG
CSB-EP764412PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP764413PIG
CSB-EP764413PIG
CSB-BP764413PIG
CSB-MP764413PIG
CSB-EP764413PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP764414PIG
CSB-EP764414PIG
CSB-BP764414PIG
CSB-MP764414PIG
CSB-EP764414PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Protein ApaG (apaG) CSB-YP764415PIG
CSB-EP764415PIG
CSB-BP764415PIG
CSB-MP764415PIG
CSB-EP764415PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 50S ribosomal protein L21 (rplU) CSB-YP764416PIG
CSB-EP764416PIG
CSB-BP764416PIG
CSB-MP764416PIG
CSB-EP764416PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Homoserine O-succinyltransferase (metA) CSB-YP764417PIG
CSB-EP764417PIG
CSB-BP764417PIG
CSB-MP764417PIG
CSB-EP764417PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP764418PIG
CSB-EP764418PIG
CSB-BP764418PIG
CSB-MP764418PIG
CSB-EP764418PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 50S ribosomal protein L24 (rplX) CSB-YP764419PIG
CSB-EP764419PIG
CSB-BP764419PIG
CSB-MP764419PIG
CSB-EP764419PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Elongation factor Tu 1 (tuf1) CSB-YP764420PIG
CSB-EP764420PIG
CSB-BP764420PIG
CSB-MP764420PIG
CSB-EP764420PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>