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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Danio rerio DNA-directed RNA polymerase I subunit RPA43 (twistnb) CSB-YP764801DIL
CSB-EP764801DIL
CSB-BP764801DIL
CSB-MP764801DIL
CSB-EP764801DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse N-alpha-acetyltransferase 35, NatC auxiliary subunit (Naa35) CSB-YP764802MO
CSB-EP764802MO
CSB-BP764802MO
CSB-MP764802MO
CSB-EP764802MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Selenoprotein T1b (selt1b) CSB-YP764803DIL
CSB-EP764803DIL
CSB-BP764803DIL
CSB-MP764803DIL
CSB-EP764803DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein BHLHb9 (BHLHB9) CSB-YP764804HU
CSB-EP764804HU
CSB-BP764804HU
CSB-MP764804HU
CSB-EP764804HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Kelch domain-containing protein 10 (KLHDC10) CSB-YP764805HU
CSB-EP764805HU
CSB-BP764805HU
CSB-MP764805HU
CSB-EP764805HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Nuclear factor related to kappa-B-binding protein (Nfrkb), partial CSB-YP764806MO
CSB-EP764806MO
CSB-BP764806MO
CSB-MP764806MO
CSB-EP764806MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Forkhead box protein R1 (FOXR1) CSB-YP764807HU
CSB-EP764807HU
CSB-BP764807HU
CSB-MP764807HU
CSB-EP764807HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human T-cell receptor-associated transmembrane adapter 1 (TRAT1), partial CSB-YP764808HU
CSB-EP764808HU
CSB-BP764808HU
CSB-MP764808HU
CSB-EP764808HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human POM121 and ZP3 fusion protein (POMZP3) CSB-YP764809HU
CSB-EP764809HU
CSB-BP764809HU
CSB-MP764809HU
CSB-EP764809HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human WD repeat-containing protein 59 (WDR59), partial CSB-YP764810HU
CSB-EP764810HU
CSB-BP764810HU
CSB-MP764810HU
CSB-EP764810HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Amine oxidase [flavin-containing] B (MAOB), partial CSB-YP764811PI
CSB-EP764811PI
CSB-BP764811PI
CSB-MP764811PI
CSB-EP764811PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus marmoreus Conotoxin mr5.4a CSB-YP764812DWD
CSB-EP764812DWD
CSB-BP764812DWD
CSB-MP764812DWD
CSB-EP764812DWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana AP2-like ethylene-responsive transcription factor BBM (BBM), partial CSB-YP764813DOA
CSB-EP764813DOA
CSB-BP764813DOA
CSB-MP764813DOA
CSB-EP764813DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amphicarpaea bracteata Maturase K (matK), partial CSB-YP764815AGAG
CSB-EP764815AGAG
CSB-BP764815AGAG
CSB-MP764815AGAG
CSB-EP764815AGAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus miles Conotoxin Mi CSB-YP764816DWE
CSB-EP764816DWE
CSB-BP764816DWE
CSB-MP764816DWE
CSB-EP764816DWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Hydroxysteroid 11-beta-dehydrogenase 1-like protein (HSD11B1L) CSB-YP764817CH
CSB-EP764817CH
CSB-BP764817CH
CSB-MP764817CH
CSB-EP764817CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana AP2-like ethylene-responsive transcription factor SMZ (SMZ) CSB-YP764818DOA
CSB-EP764818DOA
CSB-BP764818DOA
CSB-MP764818DOA
CSB-EP764818DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fascicularis Prostaglandin E synthase 3 (PTGES3) CSB-YP764819MOV
CSB-EP764819MOV
CSB-BP764819MOV
CSB-MP764819MOV
CSB-EP764819MOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beauveria bassiana Mitogen-activated protein kinase HOG1 (HOG1) CSB-YP764820BFW
CSB-EP764820BFW
CSB-BP764820BFW
CSB-MP764820BFW
CSB-EP764820BFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Cytosolic non-specific dipeptidase (Cndp2) CSB-YP764821RA
CSB-EP764821RA
CSB-BP764821RA
CSB-MP764821RA
CSB-EP764821RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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