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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse SH2 domain-containing adapter protein B (Shb) CSB-YP764781MO
CSB-EP764781MO
CSB-BP764781MO
CSB-MP764781MO
CSB-EP764781MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Basic leucine zipper and W2 domain-containing protein 1-A (bzw1a) CSB-YP764782DIL
CSB-EP764782DIL
CSB-BP764782DIL
CSB-MP764782DIL
CSB-EP764782DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Major facilitator superfamily domain-containing protein 4 (Mfsd4), partial CSB-YP764783MO
CSB-EP764783MO
CSB-BP764783MO
CSB-MP764783MO
CSB-EP764783MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein deltex-4 (Dtx4) CSB-YP764784MO
CSB-EP764784MO
CSB-BP764784MO
CSB-MP764784MO
CSB-EP764784MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein BEX3 (Ngfrap1) CSB-YP764785RA
CSB-EP764785RA
CSB-BP764785RA
CSB-MP764785RA
CSB-EP764785RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Phosphatidylinositol 4-kinase type 2-alpha (pi4k2a) CSB-YP764786DIL
CSB-EP764786DIL
CSB-BP764786DIL
CSB-MP764786DIL
CSB-EP764786DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Probable tRNA threonylcarbamoyladenosine biosynthesis protein Osgepl1 (Osgepl1) CSB-YP764787MO
CSB-EP764787MO
CSB-BP764787MO
CSB-MP764787MO
CSB-EP764787MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse CBP80/20-dependent translation initiation factor (Ctif) CSB-YP764788MO
CSB-EP764788MO
CSB-BP764788MO
CSB-MP764788MO
CSB-EP764788MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Digestive organ expansion factor (diexf), partial CSB-YP764789DIL
CSB-EP764789DIL
CSB-BP764789DIL
CSB-MP764789DIL
CSB-EP764789DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Microtubule-associated protein RP/EB family member 3 (Mapre3) CSB-YP764790MO
CSB-EP764790MO
CSB-BP764790MO
CSB-MP764790MO
CSB-EP764790MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Keratin-associated protein 26-1 (KRTAP26-1) CSB-YP764791HU
CSB-EP764791HU
CSB-BP764791HU
CSB-MP764791HU
CSB-EP764791HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human RNA (guanine-9-)-methyltransferase domain-containing protein 3 (RG9MTD3) CSB-YP764792HU
CSB-EP764792HU
CSB-BP764792HU
CSB-MP764792HU
CSB-EP764792HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Presequence protease, mitochondrial (pitrm1), partial CSB-YP764793XBE
CSB-EP764793XBE
CSB-BP764793XBE
CSB-MP764793XBE
CSB-EP764793XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Pancreatic progenitor cell differentiation and proliferation factor A (ppdpfa) CSB-YP764794DIL
CSB-EP764794DIL
CSB-BP764794DIL
CSB-MP764794DIL
CSB-EP764794DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Cadherin-24 (Cdh24), partial CSB-YP764795MO
CSB-EP764795MO
CSB-BP764795MO
CSB-MP764795MO
CSB-EP764795MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Sodium-dependent proline transporter (Slc6a7), partial CSB-YP764796MO
CSB-EP764796MO
CSB-BP764796MO
CSB-MP764796MO
CSB-EP764796MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tetratricopeptide repeat protein 37 (TTC37), partial CSB-YP764797HU
CSB-EP764797HU
CSB-BP764797HU
CSB-MP764797HU
CSB-EP764797HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio DnaJ homolog subfamily C member 21 (dnajc21), partial CSB-YP764798DIL
CSB-EP764798DIL
CSB-BP764798DIL
CSB-MP764798DIL
CSB-EP764798DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Alpha-tubulin N-acetyltransferase (atat1) CSB-YP764799DIL
CSB-EP764799DIL
CSB-BP764799DIL
CSB-MP764799DIL
CSB-EP764799DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human UPF0547 protein C16orf87 (C16orf87) CSB-YP764800HU
CSB-EP764800HU
CSB-BP764800HU
CSB-MP764800HU
CSB-EP764800HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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