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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rat Rho guanine nucleotide exchange factor 25 (Arhgef25) CSB-YP764741RA
CSB-EP764741RA
CSB-BP764741RA
CSB-MP764741RA
CSB-EP764741RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Multivesicular body subunit 12A (Fam125a) CSB-YP764742RA
CSB-EP764742RA
CSB-BP764742RA
CSB-MP764742RA
CSB-EP764742RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Tryptophan--tRNA ligase, cytoplasmic (Wars) CSB-YP764743RA
CSB-EP764743RA
CSB-BP764743RA
CSB-MP764743RA
CSB-EP764743RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Acyl-CoA desaturase 2 (Scd2), partial CSB-YP764744RA1
CSB-EP764744RA1
CSB-BP764744RA1
CSB-MP764744RA1
CSB-EP764744RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Tumor necrosis factor alpha-induced protein 8-like protein 2 (tnfaip8l2) CSB-YP764745XBE
CSB-EP764745XBE
CSB-BP764745XBE
CSB-MP764745XBE
CSB-EP764745XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Vesicle-trafficking protein SEC22b (sec22b), partial CSB-YP764746XBF
CSB-EP764746XBF
CSB-BP764746XBF
CSB-MP764746XBF
CSB-EP764746XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Sorting and assembly machinery component 50 homolog (samm50) CSB-YP764747XBF
CSB-EP764747XBF
CSB-BP764747XBF
CSB-MP764747XBF
CSB-EP764747XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Nitric oxide synthase-interacting protein (nosip) CSB-YP764748XBF
CSB-EP764748XBF
CSB-BP764748XBF
CSB-MP764748XBF
CSB-EP764748XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Keratin, type I cytoskeletal 18 (krt18) CSB-YP764749XBF
CSB-EP764749XBF
CSB-BP764749XBF
CSB-MP764749XBF
CSB-EP764749XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Non-structural maintenance of chromosomes element 1 homolog (nsmce1) CSB-YP764750XBF
CSB-EP764750XBF
CSB-BP764750XBF
CSB-MP764750XBF
CSB-EP764750XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis 40S ribosomal protein SA (rpsa) CSB-YP764751XBF
CSB-EP764751XBF
CSB-BP764751XBF
CSB-MP764751XBF
CSB-EP764751XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Diamine acetyltransferase 2 (Sat2) CSB-YP764752MO
CSB-EP764752MO
CSB-BP764752MO
CSB-MP764752MO
CSB-EP764752MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Putative ubiquitin carboxyl-terminal hydrolase 50 (Usp50) CSB-YP764753MO
CSB-EP764753MO
CSB-BP764753MO
CSB-MP764753MO
CSB-EP764753MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein phosphatase 1 regulatory subunit 3C-B (ppp1r3cb) CSB-YP764754DIL
CSB-EP764754DIL
CSB-BP764754DIL
CSB-MP764754DIL
CSB-EP764754DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tripartite motif-containing protein 40 (TRIM40) CSB-YP764755HU
CSB-EP764755HU
CSB-BP764755HU
CSB-MP764755HU
CSB-EP764755HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ubiquitin carboxyl-terminal hydrolase 49 (Usp49) CSB-YP764756MO
CSB-EP764756MO
CSB-BP764756MO
CSB-MP764756MO
CSB-EP764756MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse DENN domain-containing protein 2C (Dennd2c), partial CSB-YP764757MO
CSB-EP764757MO
CSB-BP764757MO
CSB-MP764757MO
CSB-EP764757MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Rho guanine nucleotide exchange factor 18 (Arhgef18), partial CSB-YP764758MO
CSB-EP764758MO
CSB-BP764758MO
CSB-MP764758MO
CSB-EP764758MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein slowmo homolog 2 (Slmo2) CSB-YP764759RA
CSB-EP764759RA
CSB-BP764759RA
CSB-MP764759RA
CSB-EP764759RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein fem-1 homolog A (fem1a), partial CSB-YP764760DIL
CSB-EP764760DIL
CSB-BP764760DIL
CSB-MP764760DIL
CSB-EP764760DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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