Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Desulfovibrio vulgaris 30S ribosomal protein S20 (rpsT) CSB-YP765242DDH
CSB-EP765242DDH
CSB-BP765242DDH
CSB-MP765242DDH
CSB-EP765242DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Chaperone protein ClpB (clpB), partial CSB-YP765243DDH
CSB-EP765243DDH
CSB-BP765243DDH
CSB-MP765243DDH
CSB-EP765243DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris UPF0042 nucleotide-binding protein DVU_1631 (DVU_1631) CSB-YP765244DDH
CSB-EP765244DDH
CSB-BP765244DDH
CSB-MP765244DDH
CSB-EP765244DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris DNA ligase (ligA), partial CSB-YP765245DDH
CSB-EP765245DDH
CSB-BP765245DDH
CSB-MP765245DDH
CSB-EP765245DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris ATP-dependent protease subunit HslV (hslV) CSB-YP765246DDH
CSB-EP765246DDH
CSB-BP765246DDH
CSB-MP765246DDH
CSB-EP765246DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris D-tyrosyl-tRNA (Tyr) deacylase (dtd) CSB-YP765247DDH
CSB-EP765247DDH
CSB-BP765247DDH
CSB-MP765247DDH
CSB-EP765247DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Sec-independent protein translocase protein TatA (tatA), partial CSB-YP765248DDH
CSB-EP765248DDH
CSB-BP765248DDH
CSB-MP765248DDH
CSB-EP765248DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP765249DDH
CSB-EP765249DDH
CSB-BP765249DDH
CSB-MP765249DDH
CSB-EP765249DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 50S ribosomal protein L36 (rpmJ) CSB-YP765250DDH
CSB-EP765250DDH
CSB-BP765250DDH
CSB-MP765250DDH
CSB-EP765250DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 30S ribosomal protein S17 (rpsQ) CSB-YP765251DDH
CSB-EP765251DDH
CSB-BP765251DDH
CSB-MP765251DDH
CSB-EP765251DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 50S ribosomal protein L4 (rplD) CSB-YP765252DDH
CSB-EP765252DDH
CSB-BP765252DDH
CSB-MP765252DDH
CSB-EP765252DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP765253DDH
CSB-EP765253DDH
CSB-BP765253DDH
CSB-MP765253DDH
CSB-EP765253DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris tRNA modification GTPase MnmE (mnmE) CSB-YP765254DDH
CSB-EP765254DDH
CSB-BP765254DDH
CSB-MP765254DDH
CSB-EP765254DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Alanine racemase (alr) CSB-YP765255DDH
CSB-EP765255DDH
CSB-BP765255DDH
CSB-MP765255DDH
CSB-EP765255DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 50S ribosomal protein L21 (rplU) CSB-YP765256DDH
CSB-EP765256DDH
CSB-BP765256DDH
CSB-MP765256DDH
CSB-EP765256DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Cobalamin synthase (cobS), partial CSB-YP765257DDH1
CSB-EP765257DDH1
CSB-BP765257DDH1
CSB-MP765257DDH1
CSB-EP765257DDH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Protein grpE (grpE) CSB-YP765258DDH
CSB-EP765258DDH
CSB-BP765258DDH
CSB-MP765258DDH
CSB-EP765258DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris ATP synthase gamma chain (atpG) CSB-YP765259DDH
CSB-EP765259DDH
CSB-BP765259DDH
CSB-MP765259DDH
CSB-EP765259DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Adenosylhomocysteinase (ahcY) CSB-YP765260DDH
CSB-EP765260DDH
CSB-BP765260DDH
CSB-MP765260DDH
CSB-EP765260DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP765261DDH
CSB-EP765261DDH
CSB-BP765261DDH
CSB-MP765261DDH
CSB-EP765261DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>