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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Desulfovibrio vulgaris ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP765262DDH
CSB-EP765262DDH
CSB-BP765262DDH
CSB-MP765262DDH
CSB-EP765262DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP765263DDH
CSB-EP765263DDH
CSB-BP765263DDH
CSB-MP765263DDH
CSB-EP765263DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Asparagine--tRNA ligase (asnS) CSB-YP765264DDH
CSB-EP765264DDH
CSB-BP765264DDH
CSB-MP765264DDH
CSB-EP765264DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP765265TNS
CSB-EP765265TNS
CSB-BP765265TNS
CSB-MP765265TNS
CSB-EP765265TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Proline--tRNA ligase (proS) CSB-YP765266TNS
CSB-EP765266TNS
CSB-BP765266TNS
CSB-MP765266TNS
CSB-EP765266TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP765267TNS
CSB-EP765267TNS
CSB-BP765267TNS
CSB-MP765267TNS
CSB-EP765267TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus tRNA pseudouridine synthase B (truB) CSB-YP765268TNS
CSB-EP765268TNS
CSB-BP765268TNS
CSB-MP765268TNS
CSB-EP765268TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Lipoyl synthase (lipA) CSB-YP765269TNS
CSB-EP765269TNS
CSB-BP765269TNS
CSB-MP765269TNS
CSB-EP765269TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Formate--tetrahydrofolate ligase (fhs), partial CSB-YP765270TNS
CSB-EP765270TNS
CSB-BP765270TNS
CSB-MP765270TNS
CSB-EP765270TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Protoheme IX farnesyltransferase (ctaB), partial CSB-YP765271TNS
CSB-EP765271TNS
CSB-BP765271TNS
CSB-MP765271TNS
CSB-EP765271TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP765272TNS
CSB-EP765272TNS
CSB-BP765272TNS
CSB-MP765272TNS
CSB-EP765272TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP765273TNS
CSB-EP765273TNS
CSB-BP765273TNS
CSB-MP765273TNS
CSB-EP765273TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP765274TNS
CSB-EP765274TNS
CSB-BP765274TNS
CSB-MP765274TNS
CSB-EP765274TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS), partial CSB-YP765275TNS
CSB-EP765275TNS
CSB-BP765275TNS
CSB-MP765275TNS
CSB-EP765275TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP765276TNS
CSB-EP765276TNS
CSB-BP765276TNS
CSB-MP765276TNS
CSB-EP765276TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Phosphate acyltransferase (plsX) CSB-YP765277TNS
CSB-EP765277TNS
CSB-BP765277TNS
CSB-MP765277TNS
CSB-EP765277TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 50S ribosomal protein L24 (rplX) CSB-YP765278TNS
CSB-EP765278TNS
CSB-BP765278TNS
CSB-MP765278TNS
CSB-EP765278TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Adenylate kinase (adk) CSB-YP765279TNS
CSB-EP765279TNS
CSB-BP765279TNS
CSB-MP765279TNS
CSB-EP765279TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus S-adenosylmethionine synthase (metK) CSB-YP765280TNS
CSB-EP765280TNS
CSB-BP765280TNS
CSB-MP765280TNS
CSB-EP765280TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP765281TNS
CSB-EP765281TNS
CSB-BP765281TNS
CSB-MP765281TNS
CSB-EP765281TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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