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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 50S ribosomal protein L29 (rpmC) CSB-YP766983BIK
CSB-EP766983BIK
CSB-BP766983BIK
CSB-MP766983BIK
CSB-EP766983BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L23 (rplW) CSB-YP766984BIK
CSB-EP766984BIK
CSB-BP766984BIK
CSB-MP766984BIK
CSB-EP766984BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP766985BIK
CSB-EP766985BIK
CSB-BP766985BIK
CSB-MP766985BIK
CSB-EP766985BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Membrane protein insertase YidC (yidC), partial CSB-YP766986BIK1
CSB-EP766986BIK1
CSB-BP766986BIK1
CSB-MP766986BIK1
CSB-EP766986BIK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein smg homolog (smg) CSB-YP766987BIK
CSB-EP766987BIK
CSB-BP766987BIK
CSB-MP766987BIK
CSB-EP766987BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type IV secretion system protein ptlF homolog (ptlF) CSB-YP766988BIK
CSB-EP766988BIK
CSB-BP766988BIK
CSB-MP766988BIK
CSB-EP766988BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Large-conductance mechanosensitive channel (mscL), partial CSB-YP766989BIK1
CSB-EP766989BIK1
CSB-BP766989BIK1
CSB-MP766989BIK1
CSB-EP766989BIK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidinol dehydrogenase (hisD) CSB-YP766990BIK
CSB-EP766990BIK
CSB-BP766990BIK
CSB-MP766990BIK
CSB-EP766990BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthranilate phosphoribosyltransferase (trpD) CSB-YP766991BIK
CSB-EP766991BIK
CSB-BP766991BIK
CSB-MP766991BIK
CSB-EP766991BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase gamma chain (atpG) CSB-YP766992BIK
CSB-EP766992BIK
CSB-BP766992BIK
CSB-MP766992BIK
CSB-EP766992BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP766993BIK
CSB-EP766993BIK
CSB-BP766993BIK
CSB-MP766993BIK
CSB-EP766993BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0271 protein BPP4006 (BPP4006) CSB-YP766994BIK
CSB-EP766994BIK
CSB-BP766994BIK
CSB-MP766994BIK
CSB-EP766994BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP766995BIK
CSB-EP766995BIK
CSB-BP766995BIK
CSB-MP766995BIK
CSB-EP766995BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shikimate dehydrogenase (aroE) CSB-YP766996BIK
CSB-EP766996BIK
CSB-BP766996BIK
CSB-MP766996BIK
CSB-EP766996BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP766997BIK
CSB-EP766997BIK
CSB-BP766997BIK
CSB-MP766997BIK
CSB-EP766997BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein TolB (tolB) CSB-YP766998BIK
CSB-EP766998BIK
CSB-BP766998BIK
CSB-MP766998BIK
CSB-EP766998BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP766999BIK
CSB-EP766999BIK
CSB-BP766999BIK
CSB-MP766999BIK
CSB-EP766999BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-alanine--D-alanine ligase (ddl) CSB-YP767000BIK
CSB-EP767000BIK
CSB-BP767000BIK
CSB-MP767000BIK
CSB-EP767000BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone surA (surA) CSB-YP767001BIK
CSB-EP767001BIK
CSB-BP767001BIK
CSB-MP767001BIK
CSB-EP767001BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamine--tRNA ligase (glnS), partial CSB-YP767002BIK
CSB-EP767002BIK
CSB-BP767002BIK
CSB-MP767002BIK
CSB-EP767002BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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