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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chromobacterium violaceum 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP766323CKA
CSB-EP766323CKA
CSB-BP766323CKA
CSB-MP766323CKA
CSB-EP766323CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 30S ribosomal protein S12 (rpsL) CSB-YP766324CKA
CSB-EP766324CKA
CSB-BP766324CKA
CSB-MP766324CKA
CSB-EP766324CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 30S ribosomal protein S19 (rpsS) CSB-YP766325CKA
CSB-EP766325CKA
CSB-BP766325CKA
CSB-MP766325CKA
CSB-EP766325CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 30S ribosomal protein S14 (rpsN) CSB-YP766326CKA
CSB-EP766326CKA
CSB-BP766326CKA
CSB-MP766326CKA
CSB-EP766326CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 30S ribosomal protein S11 (rpsK) CSB-YP766327CKA
CSB-EP766327CKA
CSB-BP766327CKA
CSB-MP766327CKA
CSB-EP766327CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP766328CKA
CSB-EP766328CKA
CSB-BP766328CKA
CSB-MP766328CKA
CSB-EP766328CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Ribose-phosphate pyrophosphokinase (prs) CSB-YP766329CKA
CSB-EP766329CKA
CSB-BP766329CKA
CSB-MP766329CKA
CSB-EP766329CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum DNA ligase (ligA), partial CSB-YP766330CKA
CSB-EP766330CKA
CSB-BP766330CKA
CSB-MP766330CKA
CSB-EP766330CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Ornithine carbamoyltransferase, catabolic (arcB) CSB-YP766331CKA
CSB-EP766331CKA
CSB-BP766331CKA
CSB-MP766331CKA
CSB-EP766331CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 30S ribosomal protein S20 (rpsT) CSB-YP766332CKA
CSB-EP766332CKA
CSB-BP766332CKA
CSB-MP766332CKA
CSB-EP766332CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP766333CKA
CSB-EP766333CKA
CSB-BP766333CKA
CSB-MP766333CKA
CSB-EP766333CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP766334CKA
CSB-EP766334CKA
CSB-BP766334CKA
CSB-MP766334CKA
CSB-EP766334CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 30S ribosomal protein S6 (rpsF) CSB-YP766335CKA
CSB-EP766335CKA
CSB-BP766335CKA
CSB-MP766335CKA
CSB-EP766335CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP766336CKA
CSB-EP766336CKA
CSB-BP766336CKA
CSB-MP766336CKA
CSB-EP766336CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP766337CKA
CSB-EP766337CKA
CSB-BP766337CKA
CSB-MP766337CKA
CSB-EP766337CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum SsrA-binding protein (smpB) CSB-YP766338CKA
CSB-EP766338CKA
CSB-BP766338CKA
CSB-MP766338CKA
CSB-EP766338CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Glutamyl-Q tRNA (Asp) synthetase (gluQ) CSB-YP766339CKA
CSB-EP766339CKA
CSB-BP766339CKA
CSB-MP766339CKA
CSB-EP766339CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum HPr kinase/phosphorylase (hprK) CSB-YP766340CKA
CSB-EP766340CKA
CSB-BP766340CKA
CSB-MP766340CKA
CSB-EP766340CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP766341CKA
CSB-EP766341CKA
CSB-BP766341CKA
CSB-MP766341CKA
CSB-EP766341CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UPF0758 protein CV_3079 (CV_3079) CSB-YP766342CKA
CSB-EP766342CKA
CSB-BP766342CKA
CSB-MP766342CKA
CSB-EP766342CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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