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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Glycerol kinase (glpK) CSB-YP767524BQE
CSB-EP767524BQE
CSB-BP767524BQE
CSB-MP767524BQE
CSB-EP767524BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant FMN-dependent NADH-azoreductase 1 (azoR1) CSB-YP767525BQE
CSB-EP767525BQE
CSB-BP767525BQE
CSB-MP767525BQE
CSB-EP767525BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Undecaprenyl-diphosphatase 3 (uppP3), partial CSB-YP767526BQE1
CSB-EP767526BQE1
CSB-BP767526BQE1
CSB-MP767526BQE1
CSB-EP767526BQE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 10 kDa chaperonin (groS) CSB-YP767527BQE
CSB-EP767527BQE
CSB-BP767527BQE
CSB-MP767527BQE
CSB-EP767527BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoglucosamine mutase (glmM) CSB-YP767528BQE
CSB-EP767528BQE
CSB-BP767528BQE
CSB-MP767528BQE
CSB-EP767528BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S11 (rpsK) CSB-YP767529BQE
CSB-EP767529BQE
CSB-BP767529BQE
CSB-MP767529BQE
CSB-EP767529BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L18 (rplR) CSB-YP767530BQE
CSB-EP767530BQE
CSB-BP767530BQE
CSB-MP767530BQE
CSB-EP767530BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L16 (rplP) CSB-YP767531BQE
CSB-EP767531BQE
CSB-BP767531BQE
CSB-MP767531BQE
CSB-EP767531BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L4 (rplD) CSB-YP767532BQE
CSB-EP767532BQE
CSB-BP767532BQE
CSB-MP767532BQE
CSB-EP767532BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S12 (rpsL) CSB-YP767533BQE
CSB-EP767533BQE
CSB-BP767533BQE
CSB-MP767533BQE
CSB-EP767533BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cysteine--tRNA ligase (cysS) CSB-YP767534BQE
CSB-EP767534BQE
CSB-BP767534BQE
CSB-MP767534BQE
CSB-EP767534BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA (Ile)-lysidine synthase (tilS) CSB-YP767535BQE
CSB-EP767535BQE
CSB-BP767535BQE
CSB-MP767535BQE
CSB-EP767535BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP767536BQE
CSB-EP767536BQE
CSB-BP767536BQE
CSB-MP767536BQE
CSB-EP767536BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0133 protein BA_0020/GBAA_0020/BAS0022 (BA_0020, GBAA_0020, BAS0022) CSB-YP767537BQE
CSB-EP767537BQE
CSB-BP767537BQE
CSB-MP767537BQE
CSB-EP767537BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphopantetheine adenylyltransferase (coaD) CSB-YP767538BQE
CSB-EP767538BQE
CSB-BP767538BQE
CSB-MP767538BQE
CSB-EP767538BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP767539BQE
CSB-EP767539BQE
CSB-BP767539BQE
CSB-MP767539BQE
CSB-EP767539BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional protein pyrR (pyrR) CSB-YP767540BQE
CSB-EP767540BQE
CSB-BP767540BQE
CSB-MP767540BQE
CSB-EP767540BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydroorotate dehydrogenase B (NAD (+)), electron transfer subunit (pyrK) CSB-YP767541BQE
CSB-EP767541BQE
CSB-BP767541BQE
CSB-MP767541BQE
CSB-EP767541BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methionyl-tRNA formyltransferase (fmt) CSB-YP767542BQE
CSB-EP767542BQE
CSB-BP767542BQE
CSB-MP767542BQE
CSB-EP767542BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0122 protein BA_3984/GBAA_3984/BAS3697 (BA_3984, GBAA_3984, BAS3697) CSB-YP767543BQE
CSB-EP767543BQE
CSB-BP767543BQE
CSB-MP767543BQE
CSB-EP767543BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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