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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP768387VFE
CSB-EP768387VFE
CSB-BP768387VFE
CSB-MP768387VFE
CSB-EP768387VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Formimidoylglutamase (hutG) CSB-YP768388VFE
CSB-EP768388VFE
CSB-BP768388VFE
CSB-MP768388VFE
CSB-EP768388VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal small subunit pseudouridine synthase A (rsuA) CSB-YP768389VFE
CSB-EP768389VFE
CSB-BP768389VFE
CSB-MP768389VFE
CSB-EP768389VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidine biosynthesis bifunctional protein HisIE (hisI) CSB-YP768390VFE
CSB-EP768390VFE
CSB-BP768390VFE
CSB-MP768390VFE
CSB-EP768390VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP phosphoribosyltransferase (hisG) CSB-YP768391VFE
CSB-EP768391VFE
CSB-BP768391VFE
CSB-MP768391VFE
CSB-EP768391VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP768392VFE
CSB-EP768392VFE
CSB-BP768392VFE
CSB-MP768392VFE
CSB-EP768392VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromosome partition protein mukE (mukE) CSB-YP768393VFE
CSB-EP768393VFE
CSB-BP768393VFE
CSB-MP768393VFE
CSB-EP768393VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP768394VFE
CSB-EP768394VFE
CSB-BP768394VFE
CSB-MP768394VFE
CSB-EP768394VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP768395VFE
CSB-EP768395VFE
CSB-BP768395VFE
CSB-MP768395VFE
CSB-EP768395VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0324 membrane protein VP0936 (VP0936), partial CSB-YP768396VFE
CSB-EP768396VFE
CSB-BP768396VFE
CSB-MP768396VFE
CSB-EP768396VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trigger factor (tig) CSB-YP768397VFE
CSB-EP768397VFE
CSB-BP768397VFE
CSB-MP768397VFE
CSB-EP768397VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell division topological specificity factor (minE) CSB-YP768398VFE
CSB-EP768398VFE
CSB-BP768398VFE
CSB-MP768398VFE
CSB-EP768398VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adenylate kinase (adk) CSB-YP768399VFE
CSB-EP768399VFE
CSB-BP768399VFE
CSB-MP768399VFE
CSB-EP768399VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0319 protein VP0761 (VP0761) CSB-YP768400VFE
CSB-EP768400VFE
CSB-BP768400VFE
CSB-MP768400VFE
CSB-EP768400VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptidyl-tRNA hydrolase (pth) CSB-YP768401VFE
CSB-EP768401VFE
CSB-BP768401VFE
CSB-MP768401VFE
CSB-EP768401VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leucine--tRNA ligase (leuS), partial CSB-YP768402VFE
CSB-EP768402VFE
CSB-BP768402VFE
CSB-MP768402VFE
CSB-EP768402VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serine hydroxymethyltransferase 1 (glyA1) CSB-YP768403VFE
CSB-EP768403VFE
CSB-BP768403VFE
CSB-MP768403VFE
CSB-EP768403VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP768404VFE
CSB-EP768404VFE
CSB-BP768404VFE
CSB-MP768404VFE
CSB-EP768404VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthine phosphoribosyltransferase (gpt) CSB-YP768405VFE
CSB-EP768405VFE
CSB-BP768405VFE
CSB-MP768405VFE
CSB-EP768405VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidine--tRNA ligase (hisS) CSB-YP768406VFE
CSB-EP768406VFE
CSB-BP768406VFE
CSB-MP768406VFE
CSB-EP768406VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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