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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chlamydophila caviae UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP767564DSL
CSB-EP767564DSL
CSB-BP767564DSL
CSB-MP767564DSL
CSB-EP767564DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP767565DSL
CSB-EP767565DSL
CSB-BP767565DSL
CSB-MP767565DSL
CSB-EP767565DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP767566SNN
CSB-EP767566SNN
CSB-BP767566SNN
CSB-MP767566SNN
CSB-EP767566SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP767567NHH
CSB-EP767567NHH
CSB-BP767567NHH
CSB-MP767567NHH
CSB-EP767567NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Orotate phosphoribosyltransferase (pyrE) CSB-YP767568NHH
CSB-EP767568NHH
CSB-BP767568NHH
CSB-MP767568NHH
CSB-EP767568NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Phosphopantetheine adenylyltransferase (coaD) CSB-YP767569NHH
CSB-EP767569NHH
CSB-BP767569NHH
CSB-MP767569NHH
CSB-EP767569NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Siroheme synthase (cysG) CSB-YP767570NHH
CSB-EP767570NHH
CSB-BP767570NHH
CSB-MP767570NHH
CSB-EP767570NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP767571NHH
CSB-EP767571NHH
CSB-BP767571NHH
CSB-MP767571NHH
CSB-EP767571NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase 2 (fabZ2) CSB-YP767572ELW
CSB-EP767572ELW
CSB-BP767572ELW
CSB-MP767572ELW
CSB-EP767572ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii CCA-adding enzyme (cca) CSB-YP767573DXP
CSB-EP767573DXP
CSB-BP767573DXP
CSB-MP767573DXP
CSB-EP767573DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP767574TIW
CSB-EP767574TIW
CSB-BP767574TIW
CSB-MP767574TIW
CSB-EP767574TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP767575TIV
CSB-EP767575TIV
CSB-BP767575TIV
CSB-MP767575TIV
CSB-EP767575TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP767576SZB
CSB-EP767576SZB
CSB-BP767576SZB
CSB-MP767576SZB
CSB-EP767576SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrate lyase acyl carrier protein (citD) CSB-YP767577SZB
CSB-EP767577SZB
CSB-BP767577SZB
CSB-MP767577SZB
CSB-EP767577SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Lipoyl synthase (lipA) CSB-YP767578DSL
CSB-EP767578DSL
CSB-BP767578DSL
CSB-MP767578DSL
CSB-EP767578DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP767579DSL
CSB-EP767579DSL
CSB-BP767579DSL
CSB-MP767579DSL
CSB-EP767579DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP767580DSL
CSB-EP767580DSL
CSB-BP767580DSL
CSB-MP767580DSL
CSB-EP767580DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP767581DSL
CSB-EP767581DSL
CSB-BP767581DSL
CSB-MP767581DSL
CSB-EP767581DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae DNA mismatch repair protein MutS (mutS), partial CSB-YP767582DSL
CSB-EP767582DSL
CSB-BP767582DSL
CSB-MP767582DSL
CSB-EP767582DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae 50S ribosomal protein L9 (rplI) CSB-YP767583DSL
CSB-EP767583DSL
CSB-BP767583DSL
CSB-MP767583DSL
CSB-EP767583DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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