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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0274435 (DDB_G0274435), partial CSB-YP768146DKK
CSB-EP768146DKK
CSB-BP768146DKK
CSB-MP768146DKK
CSB-EP768146DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable polyketide synthase 17 (pks17), partial CSB-YP768147DKK
CSB-EP768147DKK
CSB-BP768147DKK
CSB-MP768147DKK
CSB-EP768147DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable serine/threonine-protein kinase sky1 (sky1), partial CSB-YP768148DKK
CSB-EP768148DKK
CSB-BP768148DKK
CSB-MP768148DKK
CSB-EP768148DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative ZDHHC-type palmitoyltransferase 2 (DDB_G0274739), partial CSB-YP768149DKK1
CSB-EP768149DKK1
CSB-BP768149DKK1
CSB-MP768149DKK1
CSB-EP768149DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Protein tssc1 homolog (tssc1) CSB-YP768150DKK
CSB-EP768150DKK
CSB-BP768150DKK
CSB-MP768150DKK
CSB-EP768150DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable polyketide synthase 9/36 (pks9), partial CSB-YP768151DKK
CSB-EP768151DKK
CSB-BP768151DKK
CSB-MP768151DKK
CSB-EP768151DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Aromatic amino acid aminotransferase DDB_G0272014 (DDB_G0272014) CSB-YP768152DKK
CSB-EP768152DKK
CSB-BP768152DKK
CSB-MP768152DKK
CSB-EP768152DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable helicase DDB_G0274399 (DDB_G0274399), partial CSB-YP768153DKK
CSB-EP768153DKK
CSB-BP768153DKK
CSB-MP768153DKK
CSB-EP768153DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Expansin-like protein 5 (expl5) CSB-YP768154DKK
CSB-EP768154DKK
CSB-BP768154DKK
CSB-MP768154DKK
CSB-EP768154DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Type-1 glutamine synthetase 1 (glnA1) CSB-YP768155DKK
CSB-EP768155DKK
CSB-BP768155DKK
CSB-MP768155DKK
CSB-EP768155DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Anaphase-promoting complex subunit 8 (anapc8), partial CSB-YP768156DKK
CSB-EP768156DKK
CSB-BP768156DKK
CSB-MP768156DKK
CSB-EP768156DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein tincar (tinc), partial CSB-YP768157DLU
CSB-EP768157DLU
CSB-BP768157DLU
CSB-MP768157DLU
CSB-EP768157DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesobuthus martensii Potassium channel toxin alpha-KTx 15.8 CSB-YP768158MRE
CSB-EP768158MRE
CSB-BP768158MRE
CSB-MP768158MRE
CSB-EP768158MRE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Target of rapamycin (tor), partial CSB-YP768159DKK
CSB-EP768159DKK
CSB-BP768159DKK
CSB-MP768159DKK
CSB-EP768159DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Hybrid signal transduction histidine kinase K (dhkK), partial CSB-YP768160DKK
CSB-EP768160DKK
CSB-BP768160DKK
CSB-MP768160DKK
CSB-EP768160DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus striatus Mu-conotoxin SIIIA CSB-YP768161DWI
CSB-EP768161DWI
CSB-BP768161DWI
CSB-MP768161DWI
CSB-EP768161DWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0275531 (DDB_G0275531) CSB-YP768162DKK
CSB-EP768162DKK
CSB-BP768162DKK
CSB-MP768162DKK
CSB-EP768162DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0275589 (DDB_G0275589) CSB-YP768163DKK
CSB-EP768163DKK
CSB-BP768163DKK
CSB-MP768163DKK
CSB-EP768163DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum HssA/B-like protein 23 (hssl23) CSB-YP768164DKK
CSB-EP768164DKK
CSB-BP768164DKK
CSB-MP768164DKK
CSB-EP768164DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum mRNA turnover protein 4 homolog (mrt4) CSB-YP768165DKK
CSB-EP768165DKK
CSB-BP768165DKK
CSB-MP768165DKK
CSB-EP768165DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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