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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas putida Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon (cheB1) CSB-YP768547FGB
CSB-EP768547FGB
CSB-BP768547FGB
CSB-MP768547FGB
CSB-EP768547FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ribonuclease HI (rnhA) CSB-YP768548FGB
CSB-EP768548FGB
CSB-BP768548FGB
CSB-MP768548FGB
CSB-EP768548FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP768549FGB
CSB-EP768549FGB
CSB-BP768549FGB
CSB-MP768549FGB
CSB-EP768549FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Na (+)/H (+) antiporter nhaB (nhaB), partial CSB-YP768550FGB
CSB-EP768550FGB
CSB-BP768550FGB
CSB-MP768550FGB
CSB-EP768550FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Protein CrcB homolog (crcB), partial CSB-YP768551FGB1
CSB-EP768551FGB1
CSB-BP768551FGB1
CSB-MP768551FGB1
CSB-EP768551FGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Molybdenum import ATP-binding protein ModC (modC) CSB-YP768552FGB
CSB-EP768552FGB
CSB-BP768552FGB
CSB-MP768552FGB
CSB-EP768552FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Putative nickel-responsive regulator (PP_3341) CSB-YP768553FGB
CSB-EP768553FGB
CSB-BP768553FGB
CSB-MP768553FGB
CSB-EP768553FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Protein CsiD (csiD) CSB-YP768554FGB
CSB-EP768554FGB
CSB-BP768554FGB
CSB-MP768554FGB
CSB-EP768554FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Undecaprenyl-diphosphatase (uppP), partial CSB-YP768555FGB1
CSB-EP768555FGB1
CSB-BP768555FGB1
CSB-MP768555FGB1
CSB-EP768555FGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Urease subunit gamma (ureA) CSB-YP768556FGB
CSB-EP768556FGB
CSB-BP768556FGB
CSB-MP768556FGB
CSB-EP768556FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida GTP cyclohydrolase 1 2 (folE2) CSB-YP768557FGB
CSB-EP768557FGB
CSB-BP768557FGB
CSB-MP768557FGB
CSB-EP768557FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Glutaminase-asparaginase (ansB) CSB-YP768558FGB
CSB-EP768558FGB
CSB-BP768558FGB
CSB-MP768558FGB
CSB-EP768558FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP768559FGB
CSB-EP768559FGB
CSB-BP768559FGB
CSB-MP768559FGB
CSB-EP768559FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP768560FGB
CSB-EP768560FGB
CSB-BP768560FGB
CSB-MP768560FGB
CSB-EP768560FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Dihydroorotate dehydrogenase (quinone) (pyrD) CSB-YP768561FGB
CSB-EP768561FGB
CSB-BP768561FGB
CSB-MP768561FGB
CSB-EP768561FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP768562FGB
CSB-EP768562FGB
CSB-BP768562FGB
CSB-MP768562FGB
CSB-EP768562FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Acyl carrier protein (acpP) CSB-YP768563FGB
CSB-EP768563FGB
CSB-BP768563FGB
CSB-MP768563FGB
CSB-EP768563FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Elongation factor P (efp) CSB-YP768564FGB
CSB-EP768564FGB
CSB-BP768564FGB
CSB-MP768564FGB
CSB-EP768564FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP768565FGB
CSB-EP768565FGB
CSB-BP768565FGB
CSB-MP768565FGB
CSB-EP768565FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UPF0270 protein PP_1747 (PP_1747) CSB-YP768566FGB
CSB-EP768566FGB
CSB-BP768566FGB
CSB-MP768566FGB
CSB-EP768566FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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