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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Oryza sativa subsp. japonica Potassium transporter 27 (HAK27), partial CSB-YP769561OFG
CSB-EP769561OFG
CSB-BP769561OFG
CSB-MP769561OFG
CSB-EP769561OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Dynamin-like protein ARC5 (ARC5), partial CSB-YP769562DOA
CSB-EP769562DOA
CSB-BP769562DOA
CSB-MP769562DOA
CSB-EP769562DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable acyl-activating enzyme 18, peroxisomal (AAE18), partial CSB-YP769563DOA
CSB-EP769563DOA
CSB-BP769563DOA
CSB-MP769563DOA
CSB-EP769563DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Gamma aminobutyrate transaminase 3, chloroplastic (GABA-TP3) CSB-YP769564SZY
CSB-EP769564SZY
CSB-BP769564SZY
CSB-MP769564SZY
CSB-EP769564SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Fructan 1-exohydrolase w1 (1-FEHw1), partial CSB-YP769565TQN
CSB-EP769565TQN
CSB-BP769565TQN
CSB-MP769565TQN
CSB-EP769565TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF017 (ERF017) CSB-YP769566DOA
CSB-EP769566DOA
CSB-BP769566DOA
CSB-MP769566DOA
CSB-EP769566DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana B3 domain-containing protein At3g18960 (At3g18960) CSB-YP769567DOA
CSB-EP769567DOA
CSB-BP769567DOA
CSB-MP769567DOA
CSB-EP769567DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Aquaporin PIP2-1 (PIP2-1), partial CSB-YP769568ZAX1
CSB-EP769568ZAX1
CSB-BP769568ZAX1
CSB-MP769568ZAX1
CSB-EP769568ZAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Mixed-linked glucan synthase 2 (CSLF2), partial CSB-YP769569OFG
CSB-EP769569OFG
CSB-BP769569OFG
CSB-MP769569OFG
CSB-EP769569OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica DEAD-box ATP-dependent RNA helicase 27 (Os03g0802700, LOC_Os03g58810), partial CSB-YP769570OFG
CSB-EP769570OFG
CSB-BP769570OFG
CSB-MP769570OFG
CSB-EP769570OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays DIBOA-glucoside dioxygenase BX6 (BX6) CSB-YP769571ZAX
CSB-EP769571ZAX
CSB-BP769571ZAX
CSB-MP769571ZAX
CSB-EP769571ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DEAD-box ATP-dependent RNA helicase 57 (RH57), partial CSB-YP769572DOA
CSB-EP769572DOA
CSB-BP769572DOA
CSB-MP769572DOA
CSB-EP769572DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable methyltransferase PMT6 (At3g10200), partial CSB-YP769573DOA
CSB-EP769573DOA
CSB-BP769573DOA
CSB-MP769573DOA
CSB-EP769573DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica PHD finger protein ALFIN-LIKE 3 (Os03g0818300, LOC_Os03g60390) CSB-YP769574OFG
CSB-EP769574OFG
CSB-BP769574OFG
CSB-MP769574OFG
CSB-EP769574OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Protein LOL4 (LOL4) CSB-YP769575OFG
CSB-EP769575OFG
CSB-BP769575OFG
CSB-MP769575OFG
CSB-EP769575OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At2g16365 (At2g16365), partial CSB-YP769576DOA
CSB-EP769576DOA
CSB-BP769576DOA
CSB-MP769576DOA
CSB-EP769576DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Zinc finger CCCH domain-containing protein 27 (FES1), partial CSB-YP769577DOA
CSB-EP769577DOA
CSB-BP769577DOA
CSB-MP769577DOA
CSB-EP769577DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable galactinol--sucrose galactosyltransferase 1 (RFS1), partial CSB-YP769578DOA
CSB-EP769578DOA
CSB-BP769578DOA
CSB-MP769578DOA
CSB-EP769578DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable mannan synthase 14 (CSLA14), partial CSB-YP769579DOA1
CSB-EP769579DOA1
CSB-BP769579DOA1
CSB-MP769579DOA1
CSB-EP769579DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana SufE-like protein, chloroplastic (EMB1374) CSB-YP769580DOA
CSB-EP769580DOA
CSB-BP769580DOA
CSB-MP769580DOA
CSB-EP769580DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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