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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Adiantum capillus-veneris NAD (P)H-quinone oxidoreductase subunit J, chloroplastic (ndhJ) CSB-YP769621AXO
CSB-EP769621AXO
CSB-BP769621AXO
CSB-MP769621AXO
CSB-EP769621AXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adiantum capillus-veneris Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP769622AXO
CSB-EP769622AXO
CSB-BP769622AXO
CSB-MP769622AXO
CSB-EP769622AXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adiantum capillus-veneris ATP synthase subunit a, chloroplastic (atpI), partial CSB-YP769623AXO1
CSB-EP769623AXO1
CSB-BP769623AXO1
CSB-MP769623AXO1
CSB-EP769623AXO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adiantum capillus-veneris Photosystem II reaction center protein K (psbK) CSB-YP769624AXO
CSB-EP769624AXO
CSB-BP769624AXO
CSB-MP769624AXO
CSB-EP769624AXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanidioschyzon merolae Photosystem II reaction center protein I (psbI), partial CSB-YP769625DZU
CSB-EP769625DZU
CSB-BP769625DZU
CSB-MP769625DZU
CSB-EP769625DZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanidioschyzon merolae Photosystem II reaction center protein J (psbJ), partial CSB-YP769626DZU
CSB-EP769626DZU
CSB-BP769626DZU
CSB-MP769626DZU
CSB-EP769626DZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanidioschyzon merolae Elongation factor Ts, chloroplastic (tsf) CSB-YP769627DZU
CSB-EP769627DZU
CSB-BP769627DZU
CSB-MP769627DZU
CSB-EP769627DZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanidioschyzon merolae Cytochrome c-550 (psbV) CSB-YP769628DZU
CSB-EP769628DZU
CSB-BP769628DZU
CSB-MP769628DZU
CSB-EP769628DZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanidioschyzon merolae 30S ribosomal protein S12, chloroplastic (rps12) CSB-YP769629DZU
CSB-EP769629DZU
CSB-BP769629DZU
CSB-MP769629DZU
CSB-EP769629DZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanidioschyzon merolae 30S ribosomal protein S5, chloroplastic (rps5) CSB-YP769630DZU
CSB-EP769630DZU
CSB-BP769630DZU
CSB-MP769630DZU
CSB-EP769630DZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanidioschyzon merolae 30S ribosomal protein S17, chloroplastic (rps17) CSB-YP769631DZU
CSB-EP769631DZU
CSB-BP769631DZU
CSB-MP769631DZU
CSB-EP769631DZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanidioschyzon merolae 50S ribosomal protein L3, chloroplastic (rpl3) CSB-YP769632DZU
CSB-EP769632DZU
CSB-BP769632DZU
CSB-MP769632DZU
CSB-EP769632DZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanidioschyzon merolae 50S ribosomal protein L20 (rpl20) CSB-YP769633DZU
CSB-EP769633DZU
CSB-BP769633DZU
CSB-MP769633DZU
CSB-EP769633DZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanidioschyzon merolae Photosystem I P700 chlorophyll a apoprotein A1 (psaA), partial CSB-YP769634DZU
CSB-EP769634DZU
CSB-BP769634DZU
CSB-MP769634DZU
CSB-EP769634DZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanidioschyzon merolae 50S ribosomal protein L1, chloroplastic (rpl1) CSB-YP769635DZU
CSB-EP769635DZU
CSB-BP769635DZU
CSB-MP769635DZU
CSB-EP769635DZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanidioschyzon merolae 60 kDa chaperonin, chloroplastic (groL), partial CSB-YP769636DZU
CSB-EP769636DZU
CSB-BP769636DZU
CSB-MP769636DZU
CSB-EP769636DZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanidioschyzon merolae Putative DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP769637DZU
CSB-EP769637DZU
CSB-BP769637DZU
CSB-MP769637DZU
CSB-EP769637DZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanidioschyzon merolae Probable RuBisCO transcriptional regulator (rbcR) CSB-YP769638DZU
CSB-EP769638DZU
CSB-BP769638DZU
CSB-MP769638DZU
CSB-EP769638DZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arctonyx collaris Cytochrome b (MT-CYB), partial CSB-YP769639AGAP
CSB-EP769639AGAP
CSB-BP769639AGAP
CSB-MP769639AGAP
CSB-EP769639AGAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scalopus aquaticus Cytochrome b (MT-CYB), partial CSB-YP769640SCAB
CSB-EP769640SCAB
CSB-BP769640SCAB
CSB-MP769640SCAB
CSB-EP769640SCAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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