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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas putida 50S ribosomal protein L17 (rplQ) CSB-YP770181FGB
CSB-EP770181FGB
CSB-BP770181FGB
CSB-MP770181FGB
CSB-EP770181FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 50S ribosomal protein L14 (rplN) CSB-YP770182FGB
CSB-EP770182FGB
CSB-BP770182FGB
CSB-MP770182FGB
CSB-EP770182FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 30S ribosomal protein S19 (rpsS) CSB-YP770183FGB
CSB-EP770183FGB
CSB-BP770183FGB
CSB-MP770183FGB
CSB-EP770183FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Elongation factor Tu-B (tufB) CSB-YP770184FGB
CSB-EP770184FGB
CSB-BP770184FGB
CSB-MP770184FGB
CSB-EP770184FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 50S ribosomal protein L10 (rplJ) CSB-YP770185FGB
CSB-EP770185FGB
CSB-BP770185FGB
CSB-MP770185FGB
CSB-EP770185FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Tyrosine--tRNA ligase (tyrS) CSB-YP770186FGB
CSB-EP770186FGB
CSB-BP770186FGB
CSB-MP770186FGB
CSB-EP770186FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Esterase EstP (estP), partial CSB-YP770187FGB
CSB-EP770187FGB
CSB-BP770187FGB
CSB-MP770187FGB
CSB-EP770187FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Protein ApaG (apaG) CSB-YP770188FGB
CSB-EP770188FGB
CSB-BP770188FGB
CSB-MP770188FGB
CSB-EP770188FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Coenzyme PQQ synthesis protein A (pqqA) CSB-YP770189FGB
CSB-EP770189FGB
CSB-BP770189FGB
CSB-MP770189FGB
CSB-EP770189FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 8-amino-7-oxononanoate synthase (bioF) CSB-YP770190FGB
CSB-EP770190FGB
CSB-BP770190FGB
CSB-MP770190FGB
CSB-EP770190FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP770191FGB
CSB-EP770191FGB
CSB-BP770191FGB
CSB-MP770191FGB
CSB-EP770191FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 33 kDa chaperonin (hslO) CSB-YP770192FGB
CSB-EP770192FGB
CSB-BP770192FGB
CSB-MP770192FGB
CSB-EP770192FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida FMN reductase (NADPH) (ssuE) CSB-YP770193FGB
CSB-EP770193FGB
CSB-BP770193FGB
CSB-MP770193FGB
CSB-EP770193FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Probable GTP-binding protein EngB (engB) CSB-YP770194FGB
CSB-EP770194FGB
CSB-BP770194FGB
CSB-MP770194FGB
CSB-EP770194FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Methionyl-tRNA formyltransferase (fmt) CSB-YP770195FGB
CSB-EP770195FGB
CSB-BP770195FGB
CSB-MP770195FGB
CSB-EP770195FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Ribonuclease P protein component (rnpA) CSB-YP770196LMS
CSB-EP770196LMS
CSB-BP770196LMS
CSB-MP770196LMS
CSB-EP770196LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase (iolG) CSB-YP770197LMS
CSB-EP770197LMS
CSB-BP770197LMS
CSB-MP770197LMS
CSB-EP770197LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum L-arabinose isomerase (araA) CSB-YP770198LMS
CSB-EP770198LMS
CSB-BP770198LMS
CSB-MP770198LMS
CSB-EP770198LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum GMP reductase (guaC) CSB-YP770199LMS
CSB-EP770199LMS
CSB-BP770199LMS
CSB-MP770199LMS
CSB-EP770199LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase 2 (gpmA2) CSB-YP770200LMS
CSB-EP770200LMS
CSB-BP770200LMS
CSB-MP770200LMS
CSB-EP770200LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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