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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas putida Protein translocase subunit SecA (secA), partial CSB-YP770161FGB
CSB-EP770161FGB
CSB-BP770161FGB
CSB-MP770161FGB
CSB-EP770161FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP770162FGB
CSB-EP770162FGB
CSB-BP770162FGB
CSB-MP770162FGB
CSB-EP770162FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP770163FGB
CSB-EP770163FGB
CSB-BP770163FGB
CSB-MP770163FGB
CSB-EP770163FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Alginate biosynthesis protein AlgK (algK) CSB-YP770164FGB
CSB-EP770164FGB
CSB-BP770164FGB
CSB-MP770164FGB
CSB-EP770164FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP770165FGB
CSB-EP770165FGB
CSB-BP770165FGB
CSB-MP770165FGB
CSB-EP770165FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Probable transcriptional regulatory protein PP_1214 (PP_1214) CSB-YP770166FGB
CSB-EP770166FGB
CSB-BP770166FGB
CSB-MP770166FGB
CSB-EP770166FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida RNA polymerase-associated protein RapA (rapA), partial CSB-YP770167FGB
CSB-EP770167FGB
CSB-BP770167FGB
CSB-MP770167FGB
CSB-EP770167FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ribonuclease T (rnt) CSB-YP770168FGB
CSB-EP770168FGB
CSB-BP770168FGB
CSB-MP770168FGB
CSB-EP770168FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP770169FGB
CSB-EP770169FGB
CSB-BP770169FGB
CSB-MP770169FGB
CSB-EP770169FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Probable cytosol aminopeptidase (pepA) CSB-YP770170FGB
CSB-EP770170FGB
CSB-BP770170FGB
CSB-MP770170FGB
CSB-EP770170FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Histidinol-phosphate aminotransferase (hisC) CSB-YP770171FGB
CSB-EP770171FGB
CSB-BP770171FGB
CSB-MP770171FGB
CSB-EP770171FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C (gatC) CSB-YP770172FGB
CSB-EP770172FGB
CSB-BP770172FGB
CSB-MP770172FGB
CSB-EP770172FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP770173FGB
CSB-EP770173FGB
CSB-BP770173FGB
CSB-MP770173FGB
CSB-EP770173FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP770174FGB
CSB-EP770174FGB
CSB-BP770174FGB
CSB-MP770174FGB
CSB-EP770174FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP770175FGB
CSB-EP770175FGB
CSB-BP770175FGB
CSB-MP770175FGB
CSB-EP770175FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Outer-membrane lipoprotein LolB (lolB) CSB-YP770176FGB
CSB-EP770176FGB
CSB-BP770176FGB
CSB-MP770176FGB
CSB-EP770176FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 50S ribosomal protein L27 (rpmA) CSB-YP770177FGB
CSB-EP770177FGB
CSB-BP770177FGB
CSB-MP770177FGB
CSB-EP770177FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Chaperone protein ClpB (clpB), partial CSB-YP770178FGB
CSB-EP770178FGB
CSB-BP770178FGB
CSB-MP770178FGB
CSB-EP770178FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ethanolamine ammonia-lyase light chain (eutC) CSB-YP770179FGB
CSB-EP770179FGB
CSB-BP770179FGB
CSB-MP770179FGB
CSB-EP770179FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Transcriptional repressor NrdR (nrdR) CSB-YP770180FGB
CSB-EP770180FGB
CSB-BP770180FGB
CSB-MP770180FGB
CSB-EP770180FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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