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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella violacea DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP770254SYX
CSB-EP770254SYX
CSB-BP770254SYX
CSB-MP770254SYX
CSB-EP770254SYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triosephosphate isomerase (tpiA) CSB-YP770255KBG
CSB-EP770255KBG
CSB-BP770255KBG
CSB-MP770255KBG
CSB-EP770255KBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agmatinase (speB) CSB-YP770256EYY
CSB-EP770256EYY
CSB-BP770256EYY
CSB-MP770256EYY
CSB-EP770256EYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease P protein component (rnpA) CSB-YP770257TDAH
CSB-EP770257TDAH
CSB-BP770257TDAH
CSB-MP770257TDAH
CSB-EP770257TDAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L34 (rpmH) CSB-YP770258TNN
CSB-EP770258TNN
CSB-BP770258TNN
CSB-MP770258TNN
CSB-EP770258TNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Expansin-B17 (EXPB17) CSB-YP770259OFG
CSB-EP770259OFG
CSB-BP770259OFG
CSB-MP770259OFG
CSB-EP770259OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana RING-H2 finger protein ATL48 (ATL48), partial CSB-YP770260DOA1
CSB-EP770260DOA1
CSB-BP770260DOA1
CSB-MP770260DOA1
CSB-EP770260DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein NLP2 (NLP2), partial CSB-YP770261DOA
CSB-EP770261DOA
CSB-BP770261DOA
CSB-MP770261DOA
CSB-EP770261DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum bulbocastanum Putative disease resistance protein RGA3 (RGA3), partial CSB-YP770262STW
CSB-EP770262STW
CSB-BP770262STW
CSB-MP770262STW
CSB-EP770262STW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GDSL esterase/lipase At1g54030 (At1g54030) CSB-YP770263DOA
CSB-EP770263DOA
CSB-BP770263DOA
CSB-MP770263DOA
CSB-EP770263DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Spindle and kinetochore-associated protein 1 homolog (Os07g0484500, LOC_Os07g30110) CSB-YP770264OFG
CSB-EP770264OFG
CSB-BP770264OFG
CSB-MP770264OFG
CSB-EP770264OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Expansin-B9 (EXPB9) CSB-YP770265OFG
CSB-EP770265OFG
CSB-BP770265OFG
CSB-MP770265OFG
CSB-EP770265OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Endoribonuclease Dicer homolog 3b (DCL3B), partial CSB-YP770266OFG
CSB-EP770266OFG
CSB-BP770266OFG
CSB-MP770266OFG
CSB-EP770266OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica BURP domain-containing protein 16 (BURP16) CSB-YP770267OFG
CSB-EP770267OFG
CSB-BP770267OFG
CSB-MP770267OFG
CSB-EP770267OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor bHLH133 (BHLH133) CSB-YP770268DOA
CSB-EP770268DOA
CSB-BP770268DOA
CSB-MP770268DOA
CSB-EP770268DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable E3 ubiquitin-protein ligase XBOS34 (XBOS34), partial CSB-YP770269OFG
CSB-EP770269OFG
CSB-BP770269OFG
CSB-MP770269OFG
CSB-EP770269OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica CBL-interacting protein kinase 29 (CIPK29) CSB-YP770270OFG
CSB-EP770270OFG
CSB-BP770270OFG
CSB-MP770270OFG
CSB-EP770270OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Metacaspase-1 (AMC1) CSB-YP770271DOA
CSB-EP770271DOA
CSB-BP770271DOA
CSB-MP770271DOA
CSB-EP770271DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Carotenoid cleavage dioxygenase 7, chloroplastic (CCD7), partial CSB-YP770272DOA
CSB-EP770272DOA
CSB-BP770272DOA
CSB-MP770272DOA
CSB-EP770272DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable inactive receptor-like kinase SSP (SSP) CSB-YP770273DOA
CSB-EP770273DOA
CSB-BP770273DOA
CSB-MP770273DOA
CSB-EP770273DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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