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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila melanogaster Transient receptor potential cation channel subfamily A member 1 (TrpA1), partial CSB-YP770334DLU
CSB-EP770334DLU
CSB-BP770334DLU
CSB-MP770334DLU
CSB-EP770334DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus radiatus Iota-conotoxin-like r11c CSB-YP770335CPD
CSB-EP770335CPD
CSB-BP770335CPD
CSB-MP770335CPD
CSB-EP770335CPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus radiatus Iota-conotoxin-like R11.12 CSB-YP770336CPD
CSB-EP770336CPD
CSB-BP770336CPD
CSB-MP770336CPD
CSB-EP770336CPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratitis capitata Ribonuclease kappa-B, partial CSB-YP770337DRF1
CSB-EP770337DRF1
CSB-BP770337DRF1
CSB-MP770337DRF1
CSB-EP770337DRF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polybia scutellaris rioplatensis Venom allergen 5 CSB-YP770338PIAJ
CSB-EP770338PIAJ
CSB-BP770338PIAJ
CSB-MP770338PIAJ
CSB-EP770338PIAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Centruroides limpidus limpidus Toxin Cll5c* CSB-YP770339CGA
CSB-EP770339CGA
CSB-BP770339CGA
CSB-MP770339CGA
CSB-EP770339CGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Calcium up-regulated protein G (cupG), partial CSB-YP770340DKK
CSB-EP770340DKK
CSB-BP770340DKK
CSB-MP770340DKK
CSB-EP770340DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Lipid phosphate phosphatase-related protein type 4 (LPPR4), partial CSB-YP770341HU1
CSB-EP770341HU1
CSB-BP770341HU1
CSB-MP770341HU1
CSB-EP770341HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative uncharacterized protein C1orf191 (C1orf191) CSB-YP770342HU
CSB-EP770342HU
CSB-BP770342HU
CSB-MP770342HU
CSB-EP770342HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human G antigen family E member 2 (PAGE2) CSB-YP770343HU
CSB-EP770343HU
CSB-BP770343HU
CSB-MP770343HU
CSB-EP770343HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human KAT8 regulatory NSL complex subunit 1 (KANSL1), partial CSB-YP770344HU
CSB-EP770344HU
CSB-BP770344HU
CSB-MP770344HU
CSB-EP770344HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human C-myc promoter-binding protein (DENND4A), partial CSB-YP770345HU
CSB-EP770345HU
CSB-BP770345HU
CSB-MP770345HU
CSB-EP770345HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human CUB and sushi domain-containing protein 2 (CSMD2), partial CSB-YP770346HU
CSB-EP770346HU
CSB-BP770346HU
CSB-MP770346HU
CSB-EP770346HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human CLIP-associating protein 1 (CLASP1), partial CSB-YP770347HU
CSB-EP770347HU
CSB-BP770347HU
CSB-MP770347HU
CSB-EP770347HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human tRNA (guanine (10)-N2)-methyltransferase homolog (TRMT11) CSB-YP770348HU
CSB-EP770348HU
CSB-BP770348HU
CSB-MP770348HU
CSB-EP770348HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human LIM and senescent cell antigen-like-containing domain protein 2 (LIMS2) CSB-YP770349HU
CSB-EP770349HU
CSB-BP770349HU
CSB-MP770349HU
CSB-EP770349HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human HEAT repeat-containing protein 3 (HEATR3) CSB-YP770350HU
CSB-EP770350HU
CSB-BP770350HU
CSB-MP770350HU
CSB-EP770350HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human AMY-1-associating protein expressed in testis 1 (AAT1) CSB-YP770351HU
CSB-EP770351HU
CSB-BP770351HU
CSB-MP770351HU
CSB-EP770351HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human MAM domain-containing glycosylphosphatidylinositol anchor protein 2 (MDGA2), partial CSB-YP770352HU
CSB-EP770352HU
CSB-BP770352HU
CSB-MP770352HU
CSB-EP770352HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein FAM19A1 (FAM19A1) CSB-YP770353HU
CSB-EP770353HU
CSB-BP770353HU
CSB-MP770353HU
CSB-EP770353HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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