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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human Hydroxysteroid 11-beta-dehydrogenase 1-like protein (HSD11B1L) CSB-YP770354HU
CSB-EP770354HU
CSB-BP770354HU
CSB-MP770354HU
CSB-EP770354HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Spermatogenesis-associated protein 19, mitochondrial (SPATA19) CSB-YP770355HU
CSB-EP770355HU
CSB-BP770355HU
CSB-MP770355HU
CSB-EP770355HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human DNA polymerase nu (POLN), partial CSB-YP770356HU
CSB-EP770356HU
CSB-BP770356HU
CSB-MP770356HU
CSB-EP770356HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Sorting nexin-20 (SNX20) CSB-YP770357HU
CSB-EP770357HU
CSB-BP770357HU
CSB-MP770357HU
CSB-EP770357HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human SLIT-ROBO Rho GTPase-activating protein 1 (SRGAP1), partial CSB-YP770358HU
CSB-EP770358HU
CSB-BP770358HU
CSB-MP770358HU
CSB-EP770358HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human General receptor for phosphoinositides 1-associated scaffold protein (GRASP) CSB-YP770359HU
CSB-EP770359HU
CSB-BP770359HU
CSB-MP770359HU
CSB-EP770359HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Notch-regulated ankyrin repeat-containing protein (NRARP) CSB-YP770360HU
CSB-EP770360HU
CSB-BP770360HU
CSB-MP770360HU
CSB-EP770360HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ras-related protein Rab-44 (RAB44) CSB-YP770361HU
CSB-EP770361HU
CSB-BP770361HU
CSB-MP770361HU
CSB-EP770361HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Pleckstrin homology domain-containing family H member 3 (PLEKHH3) CSB-YP770362HU
CSB-EP770362HU
CSB-BP770362HU
CSB-MP770362HU
CSB-EP770362HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human PX domain-containing protein kinase-like protein (PXK) CSB-YP770363HU
CSB-EP770363HU
CSB-BP770363HU
CSB-MP770363HU
CSB-EP770363HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human UPF0469 protein KIAA0907 (KIAA0907) CSB-YP770364HU
CSB-EP770364HU
CSB-BP770364HU
CSB-MP770364HU
CSB-EP770364HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transmembrane emp24 domain-containing protein 4 (TMED4), partial CSB-YP770365HU
CSB-EP770365HU
CSB-BP770365HU
CSB-MP770365HU
CSB-EP770365HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Polypeptide N-acetylgalactosaminyltransferase 5 (GALNT5), partial CSB-YP770366HU
CSB-EP770366HU
CSB-BP770366HU
CSB-MP770366HU
CSB-EP770366HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces mikatae Dihydroorotate dehydrogenase (fumarate) (URA1) CSB-YP770367SBAK
CSB-EP770367SBAK
CSB-BP770367SBAK
CSB-MP770367SBAK
CSB-EP770367SBAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica pH-response regulator protein palI/RIM9 (RIM9), partial CSB-YP770368YAP1
CSB-EP770368YAP1
CSB-BP770368YAP1
CSB-MP770368YAP1
CSB-EP770368YAP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized oxidoreductase C663.09c (SPCC663.09c) CSB-YP770369SXV
CSB-EP770369SXV
CSB-BP770369SXV
CSB-MP770369SXV
CSB-EP770369SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hypocrea jecorina Xyloglucanase (cel74a), partial CSB-YP770370HDAZ
CSB-EP770370HDAZ
CSB-BP770370HDAZ
CSB-MP770370HDAZ
CSB-EP770370HDAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Segregation and condensation protein B (scpB) CSB-YP770371SLD
CSB-EP770371SLD
CSB-BP770371SLD
CSB-MP770371SLD
CSB-EP770371SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Tyrosine recombinase XerS (xerS) CSB-YP770372SMP
CSB-EP770372SMP
CSB-BP770372SMP
CSB-MP770372SMP
CSB-EP770372SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Segregation and condensation protein B (scpB) CSB-YP770373SMH
CSB-EP770373SMH
CSB-BP770373SMH
CSB-MP770373SMH
CSB-EP770373SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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