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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nitrosomonas europaea Protein translocase subunit SecA (secA), partial CSB-YP772532NHH
CSB-EP772532NHH
CSB-BP772532NHH
CSB-MP772532NHH
CSB-EP772532NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Ribosome-binding factor A (rbfA) CSB-YP772533NHH
CSB-EP772533NHH
CSB-BP772533NHH
CSB-MP772533NHH
CSB-EP772533NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Tryptophan synthase beta chain (trpB) CSB-YP772534NHH
CSB-EP772534NHH
CSB-BP772534NHH
CSB-MP772534NHH
CSB-EP772534NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea S-adenosylmethionine synthase (metK) CSB-YP772535NHH
CSB-EP772535NHH
CSB-BP772535NHH
CSB-MP772535NHH
CSB-EP772535NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP772536NHH
CSB-EP772536NHH
CSB-BP772536NHH
CSB-MP772536NHH
CSB-EP772536NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP772537NHH
CSB-EP772537NHH
CSB-BP772537NHH
CSB-MP772537NHH
CSB-EP772537NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Dephospho-CoA kinase (coaE) CSB-YP772538NHH
CSB-EP772538NHH
CSB-BP772538NHH
CSB-MP772538NHH
CSB-EP772538NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Probable cytosol aminopeptidase (pepA) CSB-YP772539NHH
CSB-EP772539NHH
CSB-BP772539NHH
CSB-MP772539NHH
CSB-EP772539NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 50S ribosomal protein L30 (rpmD) CSB-YP772540NHH
CSB-EP772540NHH
CSB-BP772540NHH
CSB-MP772540NHH
CSB-EP772540NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 50S ribosomal protein L22 (rplV) CSB-YP772541NHH
CSB-EP772541NHH
CSB-BP772541NHH
CSB-MP772541NHH
CSB-EP772541NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Putative membrane protein insertion efficiency factor (NE0388) CSB-YP772542NHH
CSB-EP772542NHH
CSB-BP772542NHH
CSB-MP772542NHH
CSB-EP772542NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Non-canonical purine NTP pyrophosphatase (NE0277) CSB-YP772543NHH
CSB-EP772543NHH
CSB-BP772543NHH
CSB-MP772543NHH
CSB-EP772543NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Probable transcriptional regulatory protein NE0210 (NE0210) CSB-YP772544NHH
CSB-EP772544NHH
CSB-BP772544NHH
CSB-MP772544NHH
CSB-EP772544NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea ATP synthase subunit delta (atpH) CSB-YP772545NHH
CSB-EP772545NHH
CSB-BP772545NHH
CSB-MP772545NHH
CSB-EP772545NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase 1 (gpmA1) CSB-YP772546NHH
CSB-EP772546NHH
CSB-BP772546NHH
CSB-MP772546NHH
CSB-EP772546NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Nucleoside diphosphate kinase (ndk) CSB-YP772547NHH
CSB-EP772547NHH
CSB-BP772547NHH
CSB-MP772547NHH
CSB-EP772547NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP772548NHH
CSB-EP772548NHH
CSB-BP772548NHH
CSB-MP772548NHH
CSB-EP772548NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Pantothenate synthetase (panC) CSB-YP772549NHH
CSB-EP772549NHH
CSB-BP772549NHH
CSB-MP772549NHH
CSB-EP772549NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 60 kDa chaperonin (groL), partial CSB-YP772550NHH
CSB-EP772550NHH
CSB-BP772550NHH
CSB-MP772550NHH
CSB-EP772550NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Membrane protein insertase YidC (yidC), partial CSB-YP772551ELW1
CSB-EP772551ELW1
CSB-BP772551ELW1
CSB-MP772551ELW1
CSB-EP772551ELW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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