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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas syringae pv. tomato Type III secretion protein hrcQa (hrcQa) CSB-YP771738FGP
CSB-EP771738FGP
CSB-BP771738FGP
CSB-MP771738FGP
CSB-EP771738FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP771739FGP
CSB-EP771739FGP
CSB-BP771739FGP
CSB-MP771739FGP
CSB-EP771739FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato GDP-mannose 6-dehydrogenase (algD) CSB-YP771740FGP
CSB-EP771740FGP
CSB-BP771740FGP
CSB-MP771740FGP
CSB-EP771740FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Probable poly (beta-D-mannuronate) O-acetylase (algI), partial CSB-YP771741FGP
CSB-EP771741FGP
CSB-BP771741FGP
CSB-MP771741FGP
CSB-EP771741FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tomato pseudo-curly top virus Replication-associated protein (C1) CSB-YP771742TDAC
CSB-EP771742TDAC
CSB-BP771742TDAC
CSB-MP771742TDAC
CSB-EP771742TDAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tobacco rattle virus 32 kDa protein CSB-YP771743TCAL
CSB-EP771743TCAL
CSB-BP771743TCAL
CSB-MP771743TCAL
CSB-EP771743TCAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Peptidyl-tRNA hydrolase (pth) CSB-YP771744FGP
CSB-EP771744FGP
CSB-BP771744FGP
CSB-MP771744FGP
CSB-EP771744FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Sugar fermentation stimulation protein homolog (sfsA) CSB-YP771745FGP
CSB-EP771745FGP
CSB-BP771745FGP
CSB-MP771745FGP
CSB-EP771745FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato DNA gyrase inhibitor YacG (yacG) CSB-YP771746FGP
CSB-EP771746FGP
CSB-BP771746FGP
CSB-MP771746FGP
CSB-EP771746FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Turnip vein-clearing virus Replicase large subunit, partial CSB-YP771747TDAG
CSB-EP771747TDAG
CSB-BP771747TDAG
CSB-MP771747TDAG
CSB-EP771747TDAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP771748FGP
CSB-EP771748FGP
CSB-BP771748FGP
CSB-MP771748FGP
CSB-EP771748FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Ethanolamine ammonia-lyase light chain (eutC) CSB-YP771749FGP
CSB-EP771749FGP
CSB-BP771749FGP
CSB-MP771749FGP
CSB-EP771749FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 6,7-dimethyl-8-ribityllumazine synthase 1 (ribH1) CSB-YP771750FGP
CSB-EP771750FGP
CSB-BP771750FGP
CSB-MP771750FGP
CSB-EP771750FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 50S ribosomal protein L18 (rplR) CSB-YP771751FGP
CSB-EP771751FGP
CSB-BP771751FGP
CSB-MP771751FGP
CSB-EP771751FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 50S ribosomal protein L24 (rplX) CSB-YP771752FGP
CSB-EP771752FGP
CSB-BP771752FGP
CSB-MP771752FGP
CSB-EP771752FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 50S ribosomal protein L4 (rplD) CSB-YP771753FGP
CSB-EP771753FGP
CSB-BP771753FGP
CSB-MP771753FGP
CSB-EP771753FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 30S ribosomal protein S12 (rpsL) CSB-YP771754FGP
CSB-EP771754FGP
CSB-BP771754FGP
CSB-MP771754FGP
CSB-EP771754FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Type III pantothenate kinase (coaX) CSB-YP771755FGP
CSB-EP771755FGP
CSB-BP771755FGP
CSB-MP771755FGP
CSB-EP771755FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Indole-3-glycerol phosphate synthase (trpC) CSB-YP771756FGP
CSB-EP771756FGP
CSB-BP771756FGP
CSB-MP771756FGP
CSB-EP771756FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP771757FGP
CSB-EP771757FGP
CSB-BP771757FGP
CSB-MP771757FGP
CSB-EP771757FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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