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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nitrosomonas europaea Uncharacterized RNA methyltransferase NE1857 (NE1857) CSB-YP772512NHH
CSB-EP772512NHH
CSB-BP772512NHH
CSB-MP772512NHH
CSB-EP772512NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Probable chorismate--pyruvate lyase (ubiC) CSB-YP772513NHH
CSB-EP772513NHH
CSB-BP772513NHH
CSB-MP772513NHH
CSB-EP772513NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase 2 (gpmA2) CSB-YP772514NHH
CSB-EP772514NHH
CSB-BP772514NHH
CSB-MP772514NHH
CSB-EP772514NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP772515NHH
CSB-EP772515NHH
CSB-BP772515NHH
CSB-MP772515NHH
CSB-EP772515NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP772516NHH
CSB-EP772516NHH
CSB-BP772516NHH
CSB-MP772516NHH
CSB-EP772516NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Undecaprenyl pyrophosphate synthase (uppS) CSB-YP772517NHH
CSB-EP772517NHH
CSB-BP772517NHH
CSB-MP772517NHH
CSB-EP772517NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 30S ribosomal protein S16 (rpsP) CSB-YP772518NHH
CSB-EP772518NHH
CSB-BP772518NHH
CSB-MP772518NHH
CSB-EP772518NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Aspartate carbamoyltransferase (pyrB) CSB-YP772519NHH
CSB-EP772519NHH
CSB-BP772519NHH
CSB-MP772519NHH
CSB-EP772519NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Protein ApaG (apaG) CSB-YP772520NHH
CSB-EP772520NHH
CSB-BP772520NHH
CSB-MP772520NHH
CSB-EP772520NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 30S ribosomal protein S9 (rpsI) CSB-YP772521NHH
CSB-EP772521NHH
CSB-BP772521NHH
CSB-MP772521NHH
CSB-EP772521NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Ferrochelatase (hemH) CSB-YP772522NHH
CSB-EP772522NHH
CSB-BP772522NHH
CSB-MP772522NHH
CSB-EP772522NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Serine hydroxymethyltransferase (glyA) CSB-YP772523NHH
CSB-EP772523NHH
CSB-BP772523NHH
CSB-MP772523NHH
CSB-EP772523NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Protease HtpX homolog (htpX), partial CSB-YP772524NHH1
CSB-EP772524NHH1
CSB-BP772524NHH1
CSB-MP772524NHH1
CSB-EP772524NHH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Adenylosuccinate synthetase (purA) CSB-YP772525NHH
CSB-EP772525NHH
CSB-BP772525NHH
CSB-MP772525NHH
CSB-EP772525NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Queuine tRNA-ribosyltransferase (tgt) CSB-YP772526NHH
CSB-EP772526NHH
CSB-BP772526NHH
CSB-MP772526NHH
CSB-EP772526NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea UPF0145 protein NE1032 (NE1032) CSB-YP772527NHH
CSB-EP772527NHH
CSB-BP772527NHH
CSB-MP772527NHH
CSB-EP772527NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP772528NHH
CSB-EP772528NHH
CSB-BP772528NHH
CSB-MP772528NHH
CSB-EP772528NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP772529NHH
CSB-EP772529NHH
CSB-BP772529NHH
CSB-MP772529NHH
CSB-EP772529NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP772530NHH
CSB-EP772530NHH
CSB-BP772530NHH
CSB-MP772530NHH
CSB-EP772530NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea LPS-assembly protein lptD (lptD), partial CSB-YP772531NHH
CSB-EP772531NHH
CSB-BP772531NHH
CSB-MP772531NHH
CSB-EP772531NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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