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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant tRNA threonylcarbamoyladenosine biosynthesis protein RimN (rimN) CSB-YP772712SZB
CSB-EP772712SZB
CSB-BP772712SZB
CSB-MP772712SZB
CSB-EP772712SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-tagatose-1,6-bisphosphate aldolase subunit kbaZ (kbaZ) CSB-YP772713SZB
CSB-EP772713SZB
CSB-BP772713SZB
CSB-MP772713SZB
CSB-EP772713SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yqgB (yqgB) CSB-YP772714SZB
CSB-EP772714SZB
CSB-BP772714SZB
CSB-MP772714SZB
CSB-EP772714SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfate adenylyltransferase subunit 1 (cysN) CSB-YP772715SZB
CSB-EP772715SZB
CSB-BP772715SZB
CSB-MP772715SZB
CSB-EP772715SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein YgaD (ygaD) CSB-YP772716SZB
CSB-EP772716SZB
CSB-BP772716SZB
CSB-MP772716SZB
CSB-EP772716SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP772717SZB
CSB-EP772717SZB
CSB-BP772717SZB
CSB-MP772717SZB
CSB-EP772717SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA ligase (ligA), partial CSB-YP772718SZB
CSB-EP772718SZB
CSB-BP772718SZB
CSB-MP772718SZB
CSB-EP772718SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase (arnT), partial CSB-YP772719SZB
CSB-EP772719SZB
CSB-BP772719SZB
CSB-MP772719SZB
CSB-EP772719SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor P-like protein (yeiP) CSB-YP772720SZB
CSB-EP772720SZB
CSB-BP772720SZB
CSB-MP772720SZB
CSB-EP772720SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable lipid kinase YegS (yegS) CSB-YP772721SZB
CSB-EP772721SZB
CSB-BP772721SZB
CSB-MP772721SZB
CSB-EP772721SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidinol-phosphate aminotransferase (hisC) CSB-YP772722SZB
CSB-EP772722SZB
CSB-BP772722SZB
CSB-MP772722SZB
CSB-EP772722SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arginine--tRNA ligase (argS), partial CSB-YP772723SZB
CSB-EP772723SZB
CSB-BP772723SZB
CSB-MP772723SZB
CSB-EP772723SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP772724SZB
CSB-EP772724SZB
CSB-BP772724SZB
CSB-MP772724SZB
CSB-EP772724SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Electron transport complex protein RnfD (rnfD), partial CSB-YP772725SZB
CSB-EP772725SZB
CSB-BP772725SZB
CSB-MP772725SZB
CSB-EP772725SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0482 protein ynfB (ynfB) CSB-YP772726SZB
CSB-EP772726SZB
CSB-BP772726SZB
CSB-MP772726SZB
CSB-EP772726SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anti-adapter protein iraM (iraM) CSB-YP772727SZB
CSB-EP772727SZB
CSB-BP772727SZB
CSB-MP772727SZB
CSB-EP772727SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP772728SZB
CSB-EP772728SZB
CSB-BP772728SZB
CSB-MP772728SZB
CSB-EP772728SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gamma-glutamyl-gamma-aminobutyrate hydrolase (puuD) CSB-YP772729SZB
CSB-EP772729SZB
CSB-BP772729SZB
CSB-MP772729SZB
CSB-EP772729SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal large subunit pseudouridine synthase E (rluE) CSB-YP772730SZB
CSB-EP772730SZB
CSB-BP772730SZB
CSB-MP772730SZB
CSB-EP772730SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein yceI (yceI) CSB-YP772731SZB
CSB-EP772731SZB
CSB-BP772731SZB
CSB-MP772731SZB
CSB-EP772731SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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