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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ascorbate-specific phosphotransferase enzyme IIA component (ulaC) CSB-YP772752SZB
CSB-EP772752SZB
CSB-BP772752SZB
CSB-MP772752SZB
CSB-EP772752SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant PyrBI operon leader peptide (pyrL) CSB-YP772753SZB
CSB-EP772753SZB
CSB-BP772753SZB
CSB-MP772753SZB
CSB-EP772753SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome c-552 (nrfA) CSB-YP772754SZB
CSB-EP772754SZB
CSB-BP772754SZB
CSB-MP772754SZB
CSB-EP772754SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L11 (rplK) CSB-YP772755SZB
CSB-EP772755SZB
CSB-BP772755SZB
CSB-MP772755SZB
CSB-EP772755SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional activator rhaS (rhaS) CSB-YP772756SZB
CSB-EP772756SZB
CSB-BP772756SZB
CSB-MP772756SZB
CSB-EP772756SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xaa-Pro dipeptidase (pepQ) CSB-YP772757SZB
CSB-EP772757SZB
CSB-BP772757SZB
CSB-MP772757SZB
CSB-EP772757SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydroxy-acid dehydratase (ilvD), partial CSB-YP772758SZB
CSB-EP772758SZB
CSB-BP772758SZB
CSB-MP772758SZB
CSB-EP772758SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP772759SZB
CSB-EP772759SZB
CSB-BP772759SZB
CSB-MP772759SZB
CSB-EP772759SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP772760SZB
CSB-EP772760SZB
CSB-BP772760SZB
CSB-MP772760SZB
CSB-EP772760SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional regulator gadX (gadX) CSB-YP772761SZB
CSB-EP772761SZB
CSB-BP772761SZB
CSB-MP772761SZB
CSB-EP772761SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant sn-glycerol-3-phosphate-binding periplasmic protein ugpB (ugpB) CSB-YP772762SZB
CSB-EP772762SZB
CSB-BP772762SZB
CSB-MP772762SZB
CSB-EP772762SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-dehydroquinate synthase (aroB) CSB-YP772763SZB
CSB-EP772763SZB
CSB-BP772763SZB
CSB-MP772763SZB
CSB-EP772763SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative glutathione-regulated potassium-efflux system protein KefB (kefB), partial CSB-YP772764SZB
CSB-EP772764SZB
CSB-BP772764SZB
CSB-MP772764SZB
CSB-EP772764SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide deformylase (def) CSB-YP772765SZB
CSB-EP772765SZB
CSB-BP772765SZB
CSB-MP772765SZB
CSB-EP772765SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Threonine/serine transporter TdcC (tdcC), partial CSB-YP772766SZB
CSB-EP772766SZB
CSB-BP772766SZB
CSB-MP772766SZB
CSB-EP772766SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (kduI) CSB-YP772767SZB
CSB-EP772767SZB
CSB-BP772767SZB
CSB-MP772767SZB
CSB-EP772767SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD (rlmD) CSB-YP772768SZB
CSB-EP772768SZB
CSB-BP772768SZB
CSB-MP772768SZB
CSB-EP772768SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Co-chaperone protein hscB (hscB) CSB-YP772769SZB
CSB-EP772769SZB
CSB-BP772769SZB
CSB-MP772769SZB
CSB-EP772769SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP772770SZB
CSB-EP772770SZB
CSB-BP772770SZB
CSB-MP772770SZB
CSB-EP772770SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fructose-like permease IIC component (fryC), partial CSB-YP772771SZB
CSB-EP772771SZB
CSB-BP772771SZB
CSB-MP772771SZB
CSB-EP772771SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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