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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saimiri sciureus Galactoside 2-alpha-L-fucosyltransferase 1 (FUT1), partial CSB-YP772952SWD
CSB-EP772952SWD
CSB-BP772952SWD
CSB-MP772952SWD
CSB-EP772952SWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lagostrophus fasciatus Sperm protamine P1 (PRM1) CSB-YP772953LAAP
CSB-EP772953LAAP
CSB-BP772953LAAP
CSB-MP772953LAAP
CSB-EP772953LAAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Carbonic anhydrase-related protein 11 (CA11) CSB-YP772954PI
CSB-EP772954PI
CSB-BP772954PI
CSB-MP772954PI
CSB-EP772954PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bos mutus grunniens Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 (GCNT3), partial CSB-YP772955BPD
CSB-EP772955BPD
CSB-BP772955BPD
CSB-MP772955BPD
CSB-EP772955BPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Chymotrypsin-like elastase family member 1 (CELA1) CSB-YP772956DO
CSB-EP772956DO
CSB-BP772956DO
CSB-MP772956DO
CSB-EP772956DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Horse 6-phosphofructokinase, muscle type (PFKM), partial CSB-YP772957HO
CSB-EP772957HO
CSB-BP772957HO
CSB-MP772957HO
CSB-EP772957HO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Culex quinquefasciatus Acetylcholinesterase (ACE-1) CSB-YP772958DZM
CSB-EP772958DZM
CSB-BP772958DZM
CSB-MP772958DZM
CSB-EP772958DZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apis mellifera Tachykinins (Atk) CSB-YP772959DNK
CSB-EP772959DNK
CSB-BP772959DNK
CSB-MP772959DNK
CSB-EP772959DNK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Acetylcholinesterase (Ace), partial CSB-YP772960BZL
CSB-EP772960BZL
CSB-BP772960BZL
CSB-MP772960BZL
CSB-EP772960BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Eukaryotic translation initiation factor 2 subunit 1 (eif2s1) CSB-YP772961DKK
CSB-EP772961DKK
CSB-BP772961DKK
CSB-MP772961DKK
CSB-EP772961DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum CTL-like protein DDB_G0274487 (DDB_G0274487), partial CSB-YP772962DKK
CSB-EP772962DKK
CSB-BP772962DKK
CSB-MP772962DKK
CSB-EP772962DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Calcium-binding protein E (cbpE) CSB-YP772963DKK
CSB-EP772963DKK
CSB-BP772963DKK
CSB-MP772963DKK
CSB-EP772963DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Homeobox protein 2 (hbx2), partial CSB-YP772964DKK
CSB-EP772964DKK
CSB-BP772964DKK
CSB-MP772964DKK
CSB-EP772964DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial (odhB) CSB-YP772965DKK
CSB-EP772965DKK
CSB-BP772965DKK
CSB-MP772965DKK
CSB-EP772965DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable splicing factor 3A subunit 1 (sf3a1), partial CSB-YP772966DKK
CSB-EP772966DKK
CSB-BP772966DKK
CSB-MP772966DKK
CSB-EP772966DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Fructose-bisphosphate aldolase (fba) CSB-YP772967DKK
CSB-EP772967DKK
CSB-BP772967DKK
CSB-MP772967DKK
CSB-EP772967DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Amidophosphoribosyltransferase (purF) CSB-YP772968DKK
CSB-EP772968DKK
CSB-BP772968DKK
CSB-MP772968DKK
CSB-EP772968DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Component of gems protein 1 (gemin1) CSB-YP772969DKK
CSB-EP772969DKK
CSB-BP772969DKK
CSB-MP772969DKK
CSB-EP772969DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0271526 (DDB_G0271526) CSB-YP772970DKK
CSB-EP772970DKK
CSB-BP772970DKK
CSB-MP772970DKK
CSB-EP772970DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Vesicle-associated membrane protein 7B (vamp7B), partial CSB-YP772971DKK
CSB-EP772971DKK
CSB-BP772971DKK
CSB-MP772971DKK
CSB-EP772971DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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